Garry Buchko

Structural Biology of the Human High Mobility Group A (HMGA) Proteins

Lead Institution

Washington State University

Principal Investigator

Raymond Reeves

Project type

Capability Research

HMGA1 proteins play a critical role in the adaptive immune response to infectious microorganisms and viruses, affecting gene regulation that stimulates resting lymphocytes into active T-cells by…

Structural Biology of the Human High Mobility Group A (HMGA) Proteins: Characterizing the Hub of Nuclear Function

Lead Institution

Washington State University

Principal Investigator

Raymond Reeves

Project type

Capability Research

Use MS to identify sites and types of post-translational modifications of several high mobility grop proteins. Use various tandem mass spec instruments available to precisely identify specific…

Seattle Structural Genomics Center for Infectious Diseases

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

Project type

Exploratory Research

The Seattle Structural Genomics Center for Infectious Disease (SSGCID) is a consortium of four institutions (Seattle Biomedical Research Institue (SBRI), deCODE biostructures, University of…

Solution Structure of a Novel Anti-fungal Defensin, MtDEF4, from Medicago truncatula (barrel clover)

Lead Institution

Donald Danforth Plant Science Center

Principal Investigator

Thomas Smith

Project type

Limited Scope

The short-term goal of this proposal is to understand the modes of action of naturally occurring, potent, anti-fungal proteins called defensins. The long-term goal is to leverage these findings to…

Seattle Structural Genomics Center for Infectious Diseases – Year XII

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

A potential consequence of rapid climate change is the emergence of epidemic infectious zoonotic diseases as the habitats of disease carriers change to expose human populations to new infectious…

Structural Genomics: Determining the Structure of Proteins from the Infectious Agent Pseudomonas aeruginosa.

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

Project type

Capability Research

The antibiotic resistance of Pseudomonas aeruginosa in chronic infections of the lungs of Cystic Fibrosis (CF) patients remains an important health issue to more than 30,000 children and adults in…

Structural Biology of the Human High Mobility Group A (HMGA) Proteins: Characterizing the Hub of Nuclear Function

Lead Institution

Washington State University

Principal Investigator

Raymond Reeves

Project type

Capability Research

The objective of this project is to provide new insight into the structural basis for some of the many biological processes involving the mammalian HMGA1 family of "high mobility group" …

Biasing conformational mobility of unstructured proteins by macromolecular crowding

Lead Institution

University of Idaho

Principal Investigator

Gary Daughdrill

Project type

Limited Scope

Macromolecular crowding is an entropic, excluded volume effect that occurs in the presence of inert macromolecules and has both thermodynamic and hydrodynamic consequences. The primary thermodynamic…

A Systems Biology Approach to Infectious Diseases Research for EnteroPathogens

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

Develop and apply new "Systems Biology" approaches to the study of bacterial pathogen species Salmonella and Yersinia, this includes proteomics, transcriptomics, metabolomics, lipidomics,…

Solid- and solution-state NMR approaches to characterizing the structure of amelogenin.

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

Biomineralization is the process used by living organism to produce minerals with properties not always found in their equivalent inorganically produced counterparts. In vertebrates, mineralized…

Structural Biology of the Human High Mobility Group A (HMGA) Proteins: Characterizing the Hub of Nuclear Function

Lead Institution

Washington State University

Principal Investigator

Raymond Reeves

Project type

Capability Research

The objective of this project is to provide new insight into the structural basis for some of the many biological processes involving the mammalian HMGA1 family of "high mobility group" …

A Systems Biology Approach to Infectious Diseases Research for EnteroPathogens

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

Develop and apply new "Systems Biology" approaches to the study of bacterial pathogen species Salmonella and Yersinia, this includes proteomics, transcriptomics, metabolomics, lipidomics,…

Understanding structure-function relationships for developing catalysts for energy storage and production reactions.

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Wendy Shaw

Project type

Large-Scale EMSL Research

The DOE Basic Energy Sciences Energy Frontier Research Center entitled “Center for Molecular Electrocatalysis” (Bullock), the DOE Basic Energy Sciences “Modular Energy-Based Approach to Molecular…

Solution-state NMR studies of catalytic intermediates of [Fe-Fe]-hydrogenase.

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

The goal of this research proposal is to establish concrete design principles that define and control efficient proton (H+) transport for [FeFe]-hydrogenase (H2ase). Proteins are able to perform…

Structural Studies of a Novel Family of Manganese Uptake Proteins in Cyanothece Containing a Repeated Five-Residues Domain (RFR).

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

Project type

Grand Challenge

Manganese is an essential trace element that all organisms require. Often, this metal is found in the enzyme?s active site, such as in ribonucleotide reductase, manganese-catalase, and manganese…

Structural Biology of DNA Repair Proteins: The Nudix Protein Family from the Extremely Radiation-Resistant Bacterium Deinococcus radiodurans.

Lead Institution

Lawrence Berkeley National Laboratory

Principal Investigator

Stephen Holbrook

Project type

Capability Research

The bacterium Deinococcus radiodurans is able to withstand high doses of ionizing and UV radiation that are lethal to virtually all other organisms (Battista, 1997). It has been suggested that this…

Seattle Structural Genomics Center for Infectious Diseases - Year VI

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

Project type

Exploratory Research

The Seattle Structural Genomics Center for Infectious Disease (SSGCID) is funded by the National Institute of Allergy and Infectious Diseases (NIAID) to apply genome-scale approaches in solving…

Seattle Structural Genomics Center for Infectious Diseases – Year XIII

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

A potential consequence of rapid climate change is the emergence of epidemic infectious zoonotic diseases as the habitats of disease carriers change to expose human populations to new infectious…

Structural Studies of a Family of Proteins from the Diurnal Cyanobacteria Cyanothece 51142 that Contain an Unusual Repeated Five-Residues Domain (RFR).

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

Project type

Grand Challenge

As complete genome sequence information became available for many organisms, a novel family of proteins containing a tandem pentapeptide repeat was discovered that can be approximately described by…

Structural Biology of Mammalian Chromatin High Mobility Group Protein HMGA1 and UV-Damaged DNA

Lead Institution

Washington State University

Principal Investigator

Michael Smerdon

Project type

Capability Research

Chromatin high mobility group protein I (HMGA1) belongs to a family of nonhistone mammalian proteins that are thought to be important structural components affecting the conformation and function of…

Enamel biomineralization: Solution- and solid-state NMR studies of a natural protein-mineral interface

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

The protein amelogenin is the central protein that controls the formation of dental enamel, the hardest tissue in the human body. The protein orchestrates the nucleation, growth, and organization of…

A multi-pronged approach to characterizing amelogenin’s role in enamel formation

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

Biomineralization is the process used by living organism to produce minerals with properties not always found in their equivalent inorganically produced counterparts. In vertebrates, mineralized…

EMSL Science Overview

Garry Buchko

Projects

Structural Biology of the Human High Mobility Group A (HMGA) Proteins

Structural Biology of the Human High Mobility Group A (HMGA) Proteins: Characterizing the Hub of Nuclear Function

Seattle Structural Genomics Center for Infectious Diseases

Solution Structure of a Novel Anti-fungal Defensin, MtDEF4, from Medicago truncatula (barrel clover)

Seattle Structural Genomics Center for Infectious Diseases – Year XII

Structural Genomics: Determining the Structure of Proteins from the Infectious Agent Pseudomonas aeruginosa.

Structural Biology of the Human High Mobility Group A (HMGA) Proteins: Characterizing the Hub of Nuclear Function

Biasing conformational mobility of unstructured proteins by macromolecular crowding

A Systems Biology Approach to Infectious Diseases Research for EnteroPathogens

Solid- and solution-state NMR approaches to characterizing the structure of amelogenin.

Structural Biology of the Human High Mobility Group A (HMGA) Proteins: Characterizing the Hub of Nuclear Function

A Systems Biology Approach to Infectious Diseases Research for EnteroPathogens

Understanding structure-function relationships for developing catalysts for energy storage and production reactions.

Solution-state NMR studies of catalytic intermediates of [Fe-Fe]-hydrogenase.

Structural Studies of a Novel Family of Manganese Uptake Proteins in Cyanothece Containing a Repeated Five-Residues Domain (RFR).

Structural Biology of DNA Repair Proteins: The Nudix Protein Family from the Extremely Radiation-Resistant Bacterium Deinococcus radiodurans.

Seattle Structural Genomics Center for Infectious Diseases - Year VI

Seattle Structural Genomics Center for Infectious Diseases – Year XIII

Structural Studies of a Family of Proteins from the Diurnal Cyanobacteria Cyanothece 51142 that Contain an Unusual Repeated Five-Residues Domain (RFR).

Structural Biology of Mammalian Chromatin High Mobility Group Protein HMGA1 and UV-Damaged DNA

Enamel biomineralization: Solution- and solid-state NMR studies of a natural protein-mineral interface

A multi-pronged approach to characterizing amelogenin’s role in enamel formation