Garry Buchko

A Systems Biology Approach to Infectious Diseases Research for EnteroPathogens

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

Develop and apply new "Systems Biology" approaches to the study of bacterial pathogen species Salmonella and Yersinia, this includes proteomics, transcriptomics, metabolomics, lipidomics,…

Solid- and solution-state NMR approaches to characterizing the structure of amelogenin.

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

Biomineralization is the process used by living organism to produce minerals with properties not always found in their equivalent inorganically produced counterparts. In vertebrates, mineralized…

Structural Biology of the Human High Mobility Group A (HMGA) Proteins: Characterizing the Hub of Nuclear Function

Lead Institution

Washington State University

Principal Investigator

Raymond Reeves

Project type

Capability Research

The objective of this project is to provide new insight into the structural basis for some of the many biological processes involving the mammalian HMGA1 family of "high mobility group" …

A Systems Biology Approach to Infectious Diseases Research for EnteroPathogens

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

Develop and apply new "Systems Biology" approaches to the study of bacterial pathogen species Salmonella and Yersinia, this includes proteomics, transcriptomics, metabolomics, lipidomics,…

Understanding structure-function relationships for developing catalysts for energy storage and production reactions.

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Wendy Shaw

Project type

Large-Scale EMSL Research

The DOE Basic Energy Sciences Energy Frontier Research Center entitled “Center for Molecular Electrocatalysis” (Bullock), the DOE Basic Energy Sciences “Modular Energy-Based Approach to Molecular…

Solution-state NMR studies of catalytic intermediates of [Fe-Fe]-hydrogenase.

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

The goal of this research proposal is to establish concrete design principles that define and control efficient proton (H+) transport for [FeFe]-hydrogenase (H2ase). Proteins are able to perform…

Structural Studies of a Novel Family of Manganese Uptake Proteins in Cyanothece Containing a Repeated Five-Residues Domain (RFR).

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

Project type

Grand Challenge

Manganese is an essential trace element that all organisms require. Often, this metal is found in the enzyme?s active site, such as in ribonucleotide reductase, manganese-catalase, and manganese…

Structural Biology of DNA Repair Proteins: The Nudix Protein Family from the Extremely Radiation-Resistant Bacterium Deinococcus radiodurans.

Lead Institution

Lawrence Berkeley National Laboratory

Principal Investigator

Stephen Holbrook

Project type

Capability Research

The bacterium Deinococcus radiodurans is able to withstand high doses of ionizing and UV radiation that are lethal to virtually all other organisms (Battista, 1997). It has been suggested that this…

Seattle Structural Genomics Center for Infectious Diseases - Year VI

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

Project type

Exploratory Research

The Seattle Structural Genomics Center for Infectious Disease (SSGCID) is funded by the National Institute of Allergy and Infectious Diseases (NIAID) to apply genome-scale approaches in solving…

Seattle Structural Genomics Center for Infectious Diseases – Year XIII

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

A potential consequence of rapid climate change is the emergence of epidemic infectious zoonotic diseases as the habitats of disease carriers change to expose human populations to new infectious…

Structural Studies of a Family of Proteins from the Diurnal Cyanobacteria Cyanothece 51142 that Contain an Unusual Repeated Five-Residues Domain (RFR).

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

Project type

Grand Challenge

As complete genome sequence information became available for many organisms, a novel family of proteins containing a tandem pentapeptide repeat was discovered that can be approximately described by…

Structural Biology of Mammalian Chromatin High Mobility Group Protein HMGA1 and UV-Damaged DNA

Lead Institution

Washington State University

Principal Investigator

Michael Smerdon

Project type

Capability Research

Chromatin high mobility group protein I (HMGA1) belongs to a family of nonhistone mammalian proteins that are thought to be important structural components affecting the conformation and function of…

Enamel biomineralization: Solution- and solid-state NMR studies of a natural protein-mineral interface

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

The protein amelogenin is the central protein that controls the formation of dental enamel, the hardest tissue in the human body. The protein orchestrates the nucleation, growth, and organization of…

A multi-pronged approach to characterizing amelogenin’s role in enamel formation

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

Biomineralization is the process used by living organism to produce minerals with properties not always found in their equivalent inorganically produced counterparts. In vertebrates, mineralized…

Source Attribution of Biological Weapons (Ricin and Associated Metabolites) Using NMR Spectroscopy

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

Project type

Capability Research

This project will study variations in the chemical and isotopic composition of biological weapons with the goal of developing a robust forensics method for deducing the origins and relatedness of…

Seattle Structural Genomics Center for Infectious Diseases - April 1, 2009 - Oct.1 2009.

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

Project type

Exploratory Research

The Seattle Structural Genomics Center for Infectious Disease (SSGCID) is a consortium of four institutions (Seattle Biomedical Research Institue (SBRI), deCODE biostructures, University of…

SSGCID - Year VII

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

Protecting the nation through innovative scientific discovery is the mission of EMSL and the mission of The Seattle Structural Genomics Center for Infectious Disease (SSGCID). The SSGCID is funded by…

COVID-19: High-throughput SARS-CoV-2-Human protein-protein interactome and drug discovery on a nanowell array chip

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Ying Zhu

The project aims to develop a high throughput and multiplex affinity purification mass spectrometry (AP-MS) assay by seamless integration of protein microarray and nanoscale proteomics. We will…

Structural Studies of Escherichia coli formamidopyrimidine DNA N-glycosylase and its main biological substrate 8-oxoguanine

Lead Institution

University of Vermont

Principal Investigator

Susan Wallace

Project type

Capability Research

DNA repair plays a fundamental role in human health. Our genomic DNA is under constant assault by endogenous oxidative stress and by exogenous factors associated with our environment. Basic…

Structural Characterization and Interactions of Conformationally Heterogeneous States of Biomolecules

Lead Institution

Universidade Federal de Pernambuco

Principal Investigator

Thereza Soares Da Silva

Project type

Large-Scale EMSL Research

This proposal seeks computational resources for the application of long-scale and enhanced sampling techniques to characterize the inherently flexible and conformationally highly heterogeneous…

Amelogenin: Solution- and solid-state studies of a natural biomineralization process

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Wendy Shaw

The protein amelogenin is the central protein controlling the formation of dental enamel, the hardest tissue in the human body. The protein orchestrates the nucleation, growth, and organization of…

COVID-19: Seattle Structural Genomics Center for Infectious Diseases – Year XIV

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

The rapid spread of SARS-CoV-2 through-out the world and its life-changing impact on the day-to-day lives of Americans punctuate the need to be ever cautious of the emergence of new epidemic…

EMSL Science Overview

Garry Buchko

Projects

A Systems Biology Approach to Infectious Diseases Research for EnteroPathogens

Solid- and solution-state NMR approaches to characterizing the structure of amelogenin.

Structural Biology of the Human High Mobility Group A (HMGA) Proteins: Characterizing the Hub of Nuclear Function

A Systems Biology Approach to Infectious Diseases Research for EnteroPathogens

Understanding structure-function relationships for developing catalysts for energy storage and production reactions.

Solution-state NMR studies of catalytic intermediates of [Fe-Fe]-hydrogenase.

Structural Studies of a Novel Family of Manganese Uptake Proteins in Cyanothece Containing a Repeated Five-Residues Domain (RFR).

Structural Biology of DNA Repair Proteins: The Nudix Protein Family from the Extremely Radiation-Resistant Bacterium Deinococcus radiodurans.

Seattle Structural Genomics Center for Infectious Diseases - Year VI

Seattle Structural Genomics Center for Infectious Diseases – Year XIII

Structural Studies of a Family of Proteins from the Diurnal Cyanobacteria Cyanothece 51142 that Contain an Unusual Repeated Five-Residues Domain (RFR).

Structural Biology of Mammalian Chromatin High Mobility Group Protein HMGA1 and UV-Damaged DNA

Enamel biomineralization: Solution- and solid-state NMR studies of a natural protein-mineral interface

A multi-pronged approach to characterizing amelogenin’s role in enamel formation

Source Attribution of Biological Weapons (Ricin and Associated Metabolites) Using NMR Spectroscopy

Seattle Structural Genomics Center for Infectious Diseases - April 1, 2009 - Oct.1 2009.

SSGCID - Year VII

COVID-19: High-throughput SARS-CoV-2-Human protein-protein interactome and drug discovery on a nanowell array chip

Structural Studies of Escherichia coli formamidopyrimidine DNA N-glycosylase and its main biological substrate 8-oxoguanine

Structural Characterization and Interactions of Conformationally Heterogeneous States of Biomolecules

Amelogenin: Solution- and solid-state studies of a natural biomineralization process

COVID-19: Seattle Structural Genomics Center for Infectious Diseases – Year XIV