Jon Magnuson

Systematic characterization of heterologous protein secretion in Aspergillus niger

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Jon Magnuson

Project type

Large-Scale EMSL Research

Economical and sustainable production of biofuels and bioproducts from biomass requires efficient utilization of the carbon contained within, thereby creating a need for enzyme cocktails used in the…

Agile BioFoundry

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Jon Magnuson

This proposed EMSL Resource Owner project, is a sub-project supporting DOE-EERE-BETO supported PNNL-national laboratory Agile BioFoundry (ABF) project. PNNL’s effort for this project, which includes…

Omics Data Integration via Interpretable Machine Learning Models

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Lisa Bramer

Often, the ultimate motivation for the generation of omics data for an experiment is a better understanding of the biological system; the generation of multiple 'omics datasets for the same…

Characterization of Application-Relevant Biosilica-Precipitating Peptides of a Diatom

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Guri Roesijadi

Project type

Large-Scale EMSL Research

NATURE OF PROPOSAL: Call for proposal- Science Theme Biological Interactions and Dynamics Type of proposal- General Type of Access- Standard Proprietary status- Nonproprietary Diatoms have…

Identification of Functional Proteins Relevant to Bioenergy and Disease Pathology by Multiplexed Activity-Based Protein Profiling

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Aaron Wright

Develop multiplexed activity-based protein profiling and apply to identification of P450s in Mycobacterium tuberculosis and biomarkers in Aspergillus fumigatus. Additionally the technology will be…

Comparative metaproteomics and metametabolomics of the biomass-degrading microbial community that inhabits the cow rumen

Lead Institution

University of California, Davis

Principal Investigator

Matthias Hess

Project type

Large-Scale EMSL Research

Lignocellulosic plant material is one of the most promising sources of sustainable liquid transportation fuels but the recalcitrant nature of the plant cell wall and the cost of biomass-degrading…

Developing Ionic-Liquid Tolerant Enzymatic Cocktails for Lignocellulosic Biomass Deconstruction

Lead Institution

Lawrence Berkeley National Laboratory

Principal Investigator

Steven Singer

Project type

Large-Scale EMSL Research

The DOE Joint BioEnergy Institute (JBEI) is tasked with developing these advanced biofuels from lignocellulosic biomass. Part of this mission is to develop efficient methods to deconstruct biomass,…

Elucidating the Effects of Gene and Protein Structure on Heterologous Expression and Secretion of Biomass Hydrolyzing Enzymes in Aspergillus niger

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Jon Magnuson

Project type

Large-Scale EMSL Research

Filamentous fungi are excellent protein production hosts as evidenced by their wide use in industry to produce a variety of native proteins. However, consistent high titer heterologous protein…

NMR identification of sugars secreted by the oleaginous yeast Lipomyces starkeyi

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Jon Magnuson

Project type

Limited Scope

Oleaginous yeasts, such as Lipomyces starkeyi, have the potential to convert sugars from lignocellulosic biomass to lipids which are ideal precursors for catalytic conversion to hydrocarbon biofuels…

Elucidating the Influences of Engineered N-glycosylation Motifs in Bacterial Biomass Hydrolyzing Enzymes upon Heterologous and Native Gene Expression, Secretion and Degradation in Aspergillus niger

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Jon Magnuson

Project type

FICUS Research

Aspergillus niger is a genetically tractable model organism for scientific discovery and a platform organism used in industry for the production of enzymes for biomass degradation and other…

Enabling Agile Biomanufacturing with Leading Edge Analytical Biology

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Mark Butcher

This proposed EMSL User project supports a larger multi-lab "Agile Biomanufacturing" project (LBNL, PNNL, SNL). Through the EMSL’s leading edge analytical biology, GC and LC mass…

Agile BioFoundry

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Jon Magnuson

This proposed EMSL Resource Owner project, is a sub-project supporting two DOE-EERE-BETO supported PNNL projects: FY16-17 four-national laboratory “Agile BioFoundry” (ABF) seed project and a larger…

Agile BioFoundry

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Kristin Burnum-Johnson

This proposed EMSL Resource Owner project, is a sub-project supporting two DOE-EERE-BETO supported PNNL projects: FY16-17 four-national laboratory “Agile BioFoundry” (ABF) seed project and a larger…

Agile BioFoundry

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Scott Baker

This proposed EMSL Resource Owner project, is a sub-project supporting two DOE-EERE-BETO supported PNNL projects: FY16-17 four-national laboratory “Agile BioFoundry” (ABF) seed project and a larger…

EMSL Science Overview

Jon Magnuson

Projects

Systematic characterization of heterologous protein secretion in Aspergillus niger

Agile BioFoundry

Omics Data Integration via Interpretable Machine Learning Models

Characterization of Application-Relevant Biosilica-Precipitating Peptides of a Diatom

Identification of Functional Proteins Relevant to Bioenergy and Disease Pathology by Multiplexed Activity-Based Protein Profiling

Comparative metaproteomics and metametabolomics of the biomass-degrading microbial community that inhabits the cow rumen

Developing Ionic-Liquid Tolerant Enzymatic Cocktails for Lignocellulosic Biomass Deconstruction

Elucidating the Effects of Gene and Protein Structure on Heterologous Expression and Secretion of Biomass Hydrolyzing Enzymes in Aspergillus niger

NMR identification of sugars secreted by the oleaginous yeast Lipomyces starkeyi

Elucidating the Influences of Engineered N-glycosylation Motifs in Bacterial Biomass Hydrolyzing Enzymes upon Heterologous and Native Gene Expression, Secretion and Degradation in Aspergillus niger

Enabling Agile Biomanufacturing with Leading Edge Analytical Biology

Agile BioFoundry

Agile BioFoundry

Agile BioFoundry