Microscopy

Advancement in energy, environment and biology research relies heavily on micro-, nano- and atomic-scale chemical and structural imaging. Many microscopy instruments have high-resolution imaging capabilities including complementary chemical, structural and phase information, in-situ imaging in native environments and imaging of dynamic processes with high temporal-resolution. See a complete list of Microscopy instruments.

Resources and Techniques

  • Nanoscale and sub-nanoscale imaging allows users to elucidate chemical processes and acquire structural data for a variety of samples such as nanostructures and cell-surface proteins.
  • Tomography yields three-dimensional reconstruction of transmission electron microscopy images generated for biological samples as well as for soft materials and samples with 3D structural heterogeneity.
  • Environmental particle analysis offers knowledge about non-volatile atmospheric particle composition and hydration properties using high-pressure scanning electron microscopy equipped with energy-dispersive x-ray analysis capability.
  • Environmental mode imaging techniques enable sample preservation to eliminate extensive preparation procedures that can introduce artifacts and make possible live-cell imaging and in situ imaging in liquids or controlled gas environments with high resolution microscopy.
  • Dynamic imaging capability enables real-time studies of nanosecond-scale dynamic processes with unprecedented spatial resolution, such as protein-protein interactions, with contrast at the single-molecule level.

Quiet Wing for Advanced Microscopy
Seven microscopes are housed in the Quiet Wing, a space specially designed to reduce external factors, such as vibrations and electromagnetic fields, that can impede capture of high-resolution images. Read more about the Quiet Wing and its instrumentation.

A new DTEM – Dynamic Transmission Electron Microscope – is under development at EMSL in collaboration with scientific colleagues at Pacific Northwest National Laboratory. It will be housed in the Quiet Wing. To learn more about this system, the science it will advance and its historical development, visit the DTEM page.

Description

Capability Details

• Electron microscopes with tomography, cryo, scanning, photoemission and high-resolution (sub-nanometer) imaging capabilities
• Focused ion beam/scanning electron microscopes for specialized sample preparation and three-dimensional topographic and chemical imaging
• Nuclear magnetic resonance microscopy with 10-40-_m resolution to study the anatomy, metabolism and transport processes of live cell cultures, biofilms and tissue samples
• Dual Raman confocal microscope for analysis of radiological samples
• Single-molecule fluorescence tools to study molecular interactions in real time
• Scanning probe microscopy with capabilities ranging from examination of dynamic nanoscale processes in condensed environments to high resolution studies of catalysis materials in ultra-high vacuum.

 

Instruments

The LEAP® 4000 XHR local electrode atom probe tomography instrument enabled the first-ever comprehensive and accurate 3-D chemical imaging studies...
Custodian(s): Arun Devaraj
This FEI Tecnai T-12 cryo-transmission electron microscope (TEM) complements EMSL's broader microscopy suite and JEOL 2010 analytical high-...
Custodian(s): Alice Dohnalkova
The Asylum MFP-3D BIO is a versatile atomic force microscope (AFM) that combines molecular resolution imaging and picoNewton force-based...
Custodian(s): Kevin M. Rosso
The Dynamic Transmission Electron Microscope, or DTEM, is under development at EMSL in collaboration with scientific colleagues at Pacific Northwest...
Housed in EMSL's RadEMSL (Radiochemistry Annex), the field emission electron microprobe (EMP) enables chemical analysis and imaging of radionuclides...
Custodian(s): Bruce Arey

Publications

A method is introduced for simple calculation of charge transfer between very large solvated organic dimers (fullerenes here) from isolated dimer...
Polarization-induced weak ferromagnetism has been predicted a few years back in perovskite MTiO3 (M = Fe, Mn, Ni) [Fennie, Phys. Rev. Lett. 100,...
Spectrum-processing software that incorporates a gaussian smoothing kernel within the statistics of first-order Kalman filtration has been developed...
LiFePO4 is a high power rate cathode material for lithium ion battery and shows remarkable capacity retention, featuring a 91% capacity retention...
The unstable interface of lithium metal in high energy density Li sulfur (Li–S) batteries raises concerns of poor cycling, low efficiency...

Science Highlights

Posted: March 02, 2017
 Frances White, PNNL, (509) 375-6904 Scientists have found adding a pinch of something new to a battery's electrolyte gives the energy storage...
Posted: March 01, 2017
The Science Lithium metal has long been considered one of the most attractive anodes for high-energy rechargeable batteries, but large-scale...
Posted: February 21, 2017
How water breaks when it hits a metal oxide surface, such as a catalyst, is important. In a pioneering study, scientists at Pacific Northwest...
Posted: February 03, 2017
The Science Photosynthetic bacteria are major primary producers on Earth, using sunlight to convert inorganic compounds in the environment into more...
Posted: January 27, 2017
Researchers at Pacific Northwest National Laboratory used EMSL capabilities to develop a "one-pot" method to produce complex, well-structured...

Instruments

Electrical, optical and mechanical properties of inorganic nanostructures have strong relationships with their morphologies. For example, one-...
The interaction between plants and their environment is of key importance in promoting sustainable, healthy, and robust plant growth. Underlying the...
The aim of this proposal is to build upon our initial success in the exploration of the 3-D location and distribution of the Al and Si atoms in...
Atmospheric aerosols have a profound effect on climate by scattering and absorbing solar and terrestrial radiation. They can be predominantly...
Quantum information science is an up and coming area of research aimed to transform computing capabilities in the future. The use of quantum bits, or...

Advancement in energy, environment and biology research relies heavily on micro-, nano- and atomic-scale chemical and structural imaging. Many microscopy instruments have high-resolution imaging capabilities including complementary chemical, structural and phase information, in-situ imaging in native environments and imaging of dynamic processes with high temporal-resolution. See a complete list of Microscopy instruments.

Resources and Techniques

  • Nanoscale and sub-nanoscale imaging allows users to elucidate chemical processes and acquire structural data for a variety of samples such as nanostructures and cell-surface proteins.
  • Tomography yields three-dimensional reconstruction of transmission electron microscopy images generated for biological samples as well as for soft materials and samples with 3D structural heterogeneity.
  • Environmental particle analysis offers knowledge about non-volatile atmospheric particle composition and hydration properties using high-pressure scanning electron microscopy equipped with energy-dispersive x-ray analysis capability.
  • Environmental mode imaging techniques enable sample preservation to eliminate extensive preparation procedures that can introduce artifacts and make possible live-cell imaging and in situ imaging in liquids or controlled gas environments with high resolution microscopy.
  • Dynamic imaging capability enables real-time studies of nanosecond-scale dynamic processes with unprecedented spatial resolution, such as protein-protein interactions, with contrast at the single-molecule level.

Quiet Wing for Advanced Microscopy
Seven microscopes are housed in the Quiet Wing, a space specially designed to reduce external factors, such as vibrations and electromagnetic fields, that can impede capture of high-resolution images. Read more about the Quiet Wing and its instrumentation.

A new DTEM – Dynamic Transmission Electron Microscope – is under development at EMSL in collaboration with scientific colleagues at Pacific Northwest National Laboratory. It will be housed in the Quiet Wing. To learn more about this system, the science it will advance and its historical development, visit the DTEM page.

Pages

Leads

(509) 371-6233

Dr. Lea provides scientific and technical expertise and leadership in support of EMSL’s user program, strategy and scientific productivity through leadership of microscopy capability and Quiet Wing for advanced microscopy. He also oversees...