Jeffrey Mclean

In Situ Magnetic Resonance Investigations of Metabolism and Mass Transport in Biofilms

Lead Institution

Washington State University

Principal Investigator

Paul Majors

Project type

Capability Research

Most microbes in nature attach to surfaces and to one another thus forming biofilms. Biofilms are diverse, robust microbiological communities that can serve beneficial (remedial ecological) or…

Interrogation of Glucose Metabolism by Oral Biofilms Using Combined NMR/Optical Spectroscopy and Stable Isotope Labeling

Lead Institution

Washington State University

Principal Investigator

Paul Majors

Project type

Capability Research

Dental caries disease and associated tooth decay (demineralization) is correlated with a decrease in pH and increase in the proportion of acidogenic and aciduric species within the attached microbial…

Interrogation of Glucose Metabolism by Oral Biofilms Using Combined NMR/Optical Spectroscopy and Stable Isotope Labeling

Lead Institution

Washington State University

Principal Investigator

Paul Majors

The overall project goal is to develop a robust technology capable of characterizing overall community metabolic function and the roles of individual species in mixed-community biofilms. This effort…

Combined NMR/Optical Microscopy for Oral Biofilm Physiology Studies

Lead Institution

Washington State University

Principal Investigator

Paul Majors

Project type

Capability Research

Dental caries disease is one of the most prevalent and costly bacterial infections in humans. Caries and associated tooth decay are strongly correlated with a pH decrease induced by oral biofilms …

PNNL Metabolomics Measurement and Validation Development for Renewable Energy Research

Lead Institution

Washington State University

Principal Investigator

Paul Majors

Project type

Capability Research

We propose the in-situ/live analysis of microbes inside of a controlled bioreactor. Further, we will determine the effects of existing extraction methods by comparing quantitative NMR metabolite…

PNNL Metabolomics Measurement and Validation Development for Renewable Energy Research

Lead Institution

Washington State University

Principal Investigator

Paul Majors

Project type

Large-Scale EMSL Research

This capability-development project will provide PNNL and DOE with a unique live / in-situ metabolomics capability for microbial bioenergy development. The technology will be applicable for the…

Integrated NMR/SIP technologies for the functional and structural characterization of microbial communities

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Johannes Scholten

Project type

Large-Scale EMSL Research

We propose to combine stable isotope probing (SIP) of DNA (SIP-DNA) with real-time on-line SIP-nuclear magnetic resonance (SIP-NMR) analysis of microbial community metabolism. SIP-NMR provides access…

COMBINED NMR/OPTICAL MICROSCOPY OF BIOFILM PHYSIOLOGY STUDIES

Lead Institution

Washington State University

Principal Investigator

Paul Majors

Project type

Large-Scale EMSL Research

Bacterial biofilms are found nearly everywhere in nature. Microbial metabolism and phenotype vary rapidly within a biofilm - this is at least partly responsible for their metabolic diversity and…

MICROSCALE METABOLIC, REDOX AND ABIOTIC REACTIONS IN HANFORD 300 AREA SUBSURFACE SEDIMENTS

Lead Institution

Washington State University

Principal Investigator

Haluk Beyenal

Project type

Exploratory Research

This proposal is the NMR component of a funded DOE/ERSP program project in which we proposed to use the Hanford 300 Area as a site for identifying microscale metabolic, abiotic and redox reactions in…

Microscale Metabolic, Redox and Abiotic Reactions in Hanford 300 Area Subsurface Sediments

Lead Institution

Washington State University

Principal Investigator

Haluk Beyenal

Project type

Large-Scale EMSL Research

This proposal is the NMR component of a funded DOE/ERSP program project in which we proposed to use the Hanford 300 Area as a site for identifying microscale metabolic, abiotic and redox reactions in…

NMR/OPTICAL Microscopy Studies of Oral Plaque Ecology

Lead Institution

University of California, Los Angeles

Principal Investigator

Wenyuan Shi

Project type

Large-Scale EMSL Research

Current tooth decay (dental caries) prevention methods include enamel hardening with fluoride and bacterial removal via mechanical and general antimicrobial approaches. These methods are based on the…

In situ monitoring of arginine in living dental biofilms

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Ryan Renslow

Dental caries disease is one of the most widespread diseases globally. A hallmark of supragingival microbial communities is the ability to rapidly metabolize carbohydrates, which dramatically reduces…

Projects