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NMR Structural Studies of Clustered DNA Damage


EMSL Project ID
10590

Abstract

The primary objective of this research is to characterize the qualitative and quantitative similarities and differences in repair characteristics between clustered damage sites formed by ionizing radiation and singly damaged sites produced by endogenous oxidative stress. Biochemical studies have shown that clustered DNA damage sites have a greater potential to contribute to the deleterious health effects of low dose radiation than single lesions. It has been shown that the structure of clustered DNA damage sites cannot be understood based on knowledge obtained from structures of single site lesions. Our goal is to determine the structure of these clustered DNA damage sites using NMR spectroscopy in order to understand the processing of these lesions by DNA repair enzymes.

Project Details

Project type
Capability Research
Start Date
2004-10-01
End Date
2005-10-03
Status
Closed

Team

Principal Investigator

Michael Kennedy
Institution
Miami University

Team Members

Kathleen McAteer
Institution
Washington State University Tri-Cities

Garry Buchko
Institution
Pacific Northwest National Laboratory

John Miller
Institution
Washington State University Tri-Cities