Identifying Targets for Therapeutic Interventions using Proteomic Technology
EMSL Project ID
10904
Abstract
This proposal will utilize new comprehensive and quantitative proteomic technologies and related capabilities developed at PNNL to identify targets for therapeutic intervention for Salmonella typhimurium, Salmonella typhi, the orthopox virus particles for vaccina, and monkey pox.Preliminary target identification from the bacteria will be the result of comparison of protein expression profiles of wild type and regulatory mutants in pure culture and in macrophage, dendritic epithelial and fibroblast host cell lines. The comparison of these profiles using bioinformatics approaches will then serve to identify protein targets important for replication and pathogenesis. Additional validation of not only the target proteins but also the bioinformatics approach will be accomplished though the screening of mutations made in target proteins.
Preliminary target identification in the virus particles will be accomplished by comparison of both variola virus (VV) and monkey pox virus (MPV) in Hela and THP-differentiated cell lines where the difference in protein expression patterns will elucidate the proteins important in pathogenesis. Once the bioinformatics approach has been demonstrated and validated with Salmonella, the approach will be extended to proteomic studies of orthopox virus particles for the early validation of the protein targets.
Project Details
Project type
Exploratory Research
Start Date
2004-09-02
End Date
2007-08-15
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members