Thrust area 1: metal-reducing Shewanella believed to be involved in the dissimilatory reduction of solid phase iron (III).
EMSL Project ID
14098
Abstract
Statement of Work to be performed at the University of East Anglia, Norwich, UKA multidisciplinary team of scientists is investigating the mechanisms of electron transfer from metal-reducing bacterial to Fe oxide surface as part of a PNNL/DOE-BER Grand Challenge in Biogeochemistry (BGC) being led by Drs. Zachara and Fredrickson of PNNL. Dr. David Richardson is one of the worlds leading experts on the biochemistry of respiratory proteins in anaerobic bacteria and has contributed to the planning phase of the grand challenge. Dr. Richardson’s participation and research efforts in the BGC are anticipated to provide key information regarding the structure and function of respiratory proteins in metal-reducing Shewanella believed to be involved in the dissimilatory reduction of solid phase iron (III).
To complete the objectives of this project, the following tasks need to be conducted at the University of East Anglia under the direction of Dr. Richardson. These tasks include:
1. Production & solution characterization of Shewanella oneidensis MR-1 proteins mtrC and mtrB and homologs in other strains of Shewanella, as necessary, whose genomes have been sequenced. Analytical methods will include, but are not limited to, EPR, redox titrations, and cyclic voltammetry.
2. EPR analyses on redox protein complexes and whole cells of MR-1 genetic constructs or deletion mutants of MR-1, and other strains of metal-reducing Shewanella. These materials will be provided either by PNNL, collaborators in the BGC, or will be generated in the PI’s laboratory.
3. Investigations into the feasibility of reconstituting electron transfer proteins into functional proteoliposomes that can facilitate EPR analyses of redox proteins & protein complexes in a lipid environment and provide relevant experimental systems for BGC members investigating fundamental mechanisms of electron transfer with metal oxides. PNNL recognizes the technical challenge associated with this task and suggests that the initial investigations be exploratory in nature. If successful, this effort may be enhanced at any point in the contract period.
Anticipated contract period: November 1, 2004 through September 30, 2007.
Project Details
Project type
Grand Challenge
Start Date
2006-07-25
End Date
2006-12-28
Status
Closed
Released Data Link
Team
Principal Investigator