Proteome analysis of regulatory gene mutants in Desulfovibrio desulfuricans G20 and Shewanella onedensis MR-1
EMSL Project ID
15902
Abstract
Sulfate-reducing and iron-reducing bacteria are important in many subsurface sediment processes. From a bioremediation standpoint, they are capable of reducing and immobilizing many toxic pollutants. Consequently, knowledge about genes and proteins that are required for survival of these organisms in-situ could enhance our understanding of and our ability to improve bioremediation processes. Signature-tagged mutagenesis was used in a DOE NABIR program supported project to identify sediment survival genes from two model environmental bacteria: Desulfovibrio desulfuricans G20 and Shewanella onedensis MR-1. Among the identified sediment survival genes, there are several annotated transcriptional regulators. In G20, transcriptional regulators include the following loci: VIMSS393448, VIMSS395152, VIMSS393140, VIMSS393918, VIMSS393957, and three histidine kinases with loci VIMSS393418, VIMSS394867, VIMSS392865 were also identified. In MR-1, transcriptional regulators annotated as HlyU (SO3538), IlvY (SO4350), a TetR family protein (SO1703), and a putative transcriptional activator (SO1985) with 28% identity to Rhodobacter sphaeroides ChrR (across the amino acid sequence) were identified. To identify genes whose expression is under the control of these regulators, we would like to compare differences in the proteome profile between these mutants and the parent strains. The parent strains were adapted to sediment conditions prior to mutagenesis procedures, and as they appear to grow more slowly in general lab media than the wild-type strains, it will also be interesting to compare their proteomes to that of wild-type G20 and MR-1. Differences in proteomes will define the roles played by these regulators in sediment survival, and we will also identify physiological changes that occur when lab strains are returned to sediment conditions.
Project Details
Project type
Exploratory Research
Start Date
2005-08-01
End Date
2006-09-06
Status
Closed
Released Data Link
Team
Principal Investigator
Related Publications
J Proteome Res. 2007 Jun 30; [Epub ahead of print] Links
Proteome Analysis of Desulfovibrio desulfuricans G20 Mutants Using the Accurate Mass and Time (AMT) Tag Approach.
Luo Q, Hixson KK, Callister SJ, Lipton MS, Morris BE, Krumholz LR.
Luo, Q., K.K. Hixson, S.J. Callister, M.S. Lipton, B. Morris and L. R. Krumholz. 2007. Proteome Analysis of Desulfovibrio desulfuricans G20 Mutants Using the Accurate Mass and Time (AMT) Tag Approach. J. Proteome Res. 6(8); 3042-3053.