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Electron Microscopy of Novel Metal-Transforming Bacteria from Extreme Environments


EMSL Project ID
16490

Abstract

Summary of Proposed Work: The Magnuson Research Group at Idaho State University has isolated and studied a number of metal-metabolizing bacteria from a variety of terrestrial habitats. Among the isolates is an arsenic-reducing thermophile, Strain YeAs. This organism is related phylogenetically to Thermobrachium, and produces a novel biogenic mineral, beta-realgar. We also have several other metal transforming organisms from acidic and high temperature environments that are under study. While we have been able to conduct a variety of experiments on these organisms, ISU does not currently have the necessary equipment or expertise in electron microscopy, a crucial technique in the study of bacterial structure and function. We propose to work with Dr. Alice Dohnalkova and staff to obtain SEM and TEM images of these interesting organisms. Of particular interest is resolution of cell wall structures, detection of extracellular proteinaceous structures such as those found in some iron-respiring organisms, and determination of cell morphology as it pertains to biofilm formation on mineral surfaces. Joint authorships on publications are expected, and it is hoped that this collaboration will lead to future research efforts between scientists at PNNL and Idaho State.

Project Details

Project type
Exploratory Research
Start Date
2006-07-24
End Date
2007-10-01
Status
Closed

Team

Principal Investigator

Timothy Magnuson
Institution
Idaho State University