Non-invasive monitoring of bFGF-induced signaling
EMSL Project ID
17393
Abstract
Basic fibroblast growth factor (bFGF)is implicated in human carcinogensis, therefore, a comprehensive understanding of the biological effects of bFGF is expected to provide opportunities to improve human health. We have been investigating the effects of bFGF on cell transformation response in vitro and are interested in determining non-invasive measurements of bFGF-dependent signaling that will enable investigation of more complex whole animal model systems. Features of our model that we believe will result in successful analysis by NMR include the high cell densities that can be used (100% of the growth surface can be covered with cells) and the high response of cells to bFGF (virtually 100% of cells respond). bFGF treated cells undergo a morphological change and we have engineered cells that express the green fluorescent protein to exploit the optical imaging component of the combinded microscope. This will provide added confidence in the interpretation of NMR data from live cell studies. We propose to define the molecular features associated with bFGF treatment using the combined microscope.
Project Details
Project type
Capability Research
Start Date
2006-12-01
End Date
2007-07-18
Status
Closed
Released Data Link
Team
Principal Investigator