Quantifying Human Liver Alcohol Dehydrogenase Isoenzymes
EMSL Project ID
1788
Abstract
Researchers report individual variation in ethanol elimination, both within and between ethnic groups, as well as between gender groups. The human alcohol dehydrogenase isoenzymes expressed in liver are believed to play a major role in ethanol elimination. As many as 17 catalytically active alcohol dehydrogenase isoenzymes are expressed in human liver. Each alcohol dehydrogenase isoenzyme may contribute to ethanol elimination, but it is not known which isoenzymes are most important in ethanol metabolism. We hypothesize that individual variation in ethanol elimination rates correlates with the differences in the amounts of the alcohol dehydrogenase isoenzymes expressed in an individual liver. The aim of this proposal is to explore methods for separating and quantifying the alcohol dehydrogenase isoenzymes from human liver. State-of-the-art techniques in HPLC, capillary electrophoresis and mass spectrometry will be used to develop the protocols. The methods developed here will allow researchers to study the correlations between the ability of an individual liver sample to eliminate ethanol and the alcohol dehydrogenase isoenzymes expressed in that liver sample
Project Details
Project type
Exploratory Research
Start Date
1999-12-29
End Date
2002-09-24
Status
Closed
Released Data Link
Team
Principal Investigator