Proteome analysis of Anaeromyxobacter dehalogenans strain 2CP-C
EMSL Project ID
19397
Abstract
Anaeromyxobacter dehalogenans is a physiologically versatile, facultatively aerobic bacterium commonly found in soils and subsurface environments. The organism reduces metals and radionuclides including uranium and technetium. Growth studies with Anaeromyxobacter dehalogenans strain 2CP-C demonstrated that U(VI) serves as metabolic electron acceptor. Further, 16S rRNA gene-based analyses detected Anaeromyxobacter species at the FRC suggesting these organisms contribute to U(VI) reduction and immobilization. The genome of strain 2CP-C has been sequenced and whole genome microarrays are currently being constructed in collaboration with PNNL (Beliaev and Fredrickson). Further, we are using MALDI TOF-MS as a tool for the specific detection of strain 2CP-C. The goal of the proteome analysis of strain 2CP-C is to generate new information to support the ongoing research efforts, to identify proteins involved in uranium reduction and other terminal electron-accepting processes, to formulate new hypotheses on the mechanisms of metal reduction, and ultimately to develop collaborative proposals exploring Anaeromyxobacter dehalogenans.
Project Details
Start Date
2006-06-01
End Date
2007-10-01
Status
Closed
Released Data Link
Team
Principal Investigator