Structural Engineering of the de novo Designed Protein Top7 as
a Scaffold for Antigen-binding

Deployable biosensors for detecting environmental contaminants at the source require molecular sensing elements or affinity reagents that can survive the fluctuations in temperature and complex samples that are encountered in the field. All-atom molecular dynamics (MD) simulations will be performed for top7 variants - 5, 10 or 15 residue insertions at each of the six loop - in different solvents (water and methanol) and at different conditions of temperature and pH.