Structural Determination of a Complex Membrane Protein, Diacylglycerol Kinase
EMSL Project ID
2173
Abstract
Prokaryotic diacylglycerol kinase (DAGK) is a homotrimeric integral membrane protein composed of 13 kDa subunits, with each subunit having 3 transmembrane helices. As a membrane protein, DAGK represents a class of proteins which is of enormous biological and biomedical importance but which has been proven largely refractory to structural determination by classical NMR and crystallographic methods. Indeed, there are presently no published reports of NMR-based structures for any >10 kDa membrane protein (although there is a rumor that a rough structure of a 17 kDa bacterial outer membrane porin solubilized in detergents may be in the pipeline.)
Project Details
Project type
Capability Research
Start Date
2001-05-30
End Date
2001-06-12
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members