ICAT labeling of P aeruginosa membrane subproteome
EMSL Project ID
2497
Abstract
Pseudomonas aeruginosa is one of the top three causes of life-threatening infections in cystic fibrosis, burn, HIV, and cancer patients. The pathogenesis of P. aeruginosa is complex. This proposed research continues our collaborative effort to study the virulent related membrane and extracellular sub-proteomes of P. aeruginosa applying the advanced proteomic methods pioneered by Dr.Smith?s lab (EMSL proposal NO. 2394. We have submitted a Statements of Intent for a new NIH program for multidisciplinary studies of pathogenesis). Specific Aim 1: Continue the identification of the accurate mass tags for the membrane and secretory proteins of P. aeruginosa. Very significant progress has been archived on the analysis of the membrane sub-proteome, showing promising AMTs for up to 900 proteins (EMSL proposal NO. 2394). To confirm and expand these AMTs, the d0/d8 ICAT labeling and LC-ITMS analysis will be performed on the membrane isolations from cells of different growth stages. Specific Aim 2: Comparative analysis of the virulent wild type and non-virulent strains due to the deletion of a virulence-related transcription factor. Results on the differences in secretory and membrane proteins, together with data from gene expression analysis using DNA microarray, will allow us to model the related regulatory circuits of the infection machinery. This example will serve as a demonstration of our multidisciplinary approach to pathogenesis study.
Project Details
Project type
Exploratory Research
Start Date
2002-04-08
End Date
2004-01-17
Status
Closed
Released Data Link
Team
Principal Investigator
Related Publications
Williams JJ, J Walters, M Wang, N Chawla, and A Rohatgi. 2013. "Extracting Constitutive Stress-Strain Behavior of Microscopic Phases by Micropillar Compression." JOM. The Journal of the Minerals, Metals and Materials Society 65(2):226-233. doi:10.1007/s11837-012-0516-9