Identification of proteins differentially expressed in response IR.
EMSL Project ID
2555
Abstract
Our laboratory has used cDNA microarray studies to identify ionizing radiation (IR) modulation transcripts for genes such as transcription factors, oncogenes, intercellular signaling factors and growth factors, as well as, genes involved in response to tissue injury, inflammation, oxidative stress, and protective responses. We are now interested in a similar approach to identify IR-responsive proteins for comaprison. Initial characterization of isolated protein extracts from IR-treated (10 cGy, 200 cGy) and untreated cells has identified several unique protein peaks that were found using SELDI mass-spectrometry. In addition, several proteins were equally expressed under all conditions. To get a better idea of the human proteome responses to IR other more scalable techniques are required. We propose to work with PNNL and Dr. David Camp to use mass-spectrometry to characterize IR induced changes at the protein level. To identify unique IR proteins we will compare IR-related responses in one Correil human cell line (15510) treated with 0 cGy, 10 cGy, 200 cGy, and an adaptive dose of 5 cGy followed by 200 cGy. Proteins will be isolated at LLNL, and adjusted to 0.1 mg/mL for subsequent spectroscopy at PNNL.
Project Details
Project type
Exploratory Research
Start Date
2002-07-01
End Date
2005-07-03
Status
Closed
Released Data Link
Team
Principal Investigator