Observation of Biogenic Nano-scaled Wire-like Arsenic Sulfide Mineral
EMSL Project ID
26711
Abstract
A facultative anaerobic rod, dissimilatory iron-reducing bacterium, Shewanella sp. strain HN-41, which was isolated from a oil shale rock fragment collected at a tidal in Haenam, South Korea, was cultured in a defined medium supplemented with DL-lactate as an electron-donor and As(V) and S2O32- as potential electron acceptors, in anaerobic condition. In the incubation, strain HN-41 was supposed to reduce both A(V) and S2O32- concomitantly to As(III) and S2-, respectively. And fiber-like and wire-like elongated arsenic sulfide (As2S3) was formed extracellularly. To look insight into the As-S materials in the mineralogical, biological, and nanomaterial view points, we want to use EMSL capabilities such as TEM tomography and High-Resolution TEM for selected area diffraction (SAD) and nanobeam diffraction (NBD) techniques, as well as direct high resolution imaging.Especially, the morphology of wire-like elongated arsenic sulfide is likely newly formed biomineral, we would like to use TEM tomography for determination of the material morphology in 3-D that will help us solve the question whether the observed structures are membrane vesicles or cross-sections of extracellular polymeric substances.
The results that would be obtained at EMSL could help to understand the biomineralization phenomenon about the arsenic sulfide, and could suggest potential applications for the biomineral, nano-scaled arsenic sulfide, and for bioremediation of arsenic in subsurface environments by precipitating toxic arsenic ions to insoluble solids.
Project Details
Project type
Capability Research
Start Date
2007-08-03
End Date
2009-08-09
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Lee JH, MG Kim, B Yoo, NV Myung, J Maeng, T Lee, A Dohnalkova, JK Fredrickson, MJ Sadowsky, and HG Hur. 2007. "Biogenic formation of photoactive arsenic-sulfide nanotubes by Shewanella sp. strain HN-41 ." Proceedings of the National Academy of Sciences of the United States of America 104(51):20410-20415. doi:10.1073/pnas.0707595104