Developing a systems biology approach for the termite gut microbial isolate Verrucomicrobium sp. strain TAV2.
EMSL Project ID
28690
Abstract
Termites are dedicated to transform cellulosic biomass into soluble oligosaccharides, H2, and methane by harboring an entire microbial community. The genome of the Verrucomicrobium sp. TAV2, a member of this community, has revealed important gene patterns consistent with its habitat of isolation and maintenance of low O2 concentration, important for the termite hindgut ecosystem stability. We hypothesize that TAV2 express key genes and pathways differentially under microoxic conditions as compared to oxic conditions. The scientific goals of this research are: 1) To identify and compare the proteomic profiles of strain TAV2 under defined O2 conditions; 2) To quantify proteomic differences responsible for maintaining the low O2 ecosystem level; and 3) To integrate the sequeced genome of TAV2 and its proteomic profile. We anticipate a two-fold use of the data by developing a guided systems biology approach for members of poorly characterized phylum as Verrucomicrobia and by providing resources for a proteomic database of a biofuel-converter microbial community.
Project Details
Project type
Exploratory Research
Start Date
2008-04-01
End Date
2009-04-05
Status
Closed
Released Data Link
Team
Principal Investigator
Related Publications
Isanapong J, S Hambright, AG Willis, A Boonmee, SJ Callister, KE Burnum-Johnson, L Pasa-Tolic, CD Nicora, JT Wertz, T Schmidt, and JL Rodrigues. 2013. "Development of an ecophysiological model for Diplosphaera colotermitum TAV2, a termite hindgut Verrucomicrobium." The ISME Journal 7(9):1803-1813. doi:10.1038/ismej.2013.74