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Proteomics Methods and QSAR models to predict nanoparticle surface chemistry interactions


EMSL Project ID
31291

Abstract

Biocompatibility of nanomaterials is now a serious bottleneck in the rapidly growing fields of nanomedicine and nanotoxicology. We propose to test the hypothesis that differences in disposition and biocompatibility of different nanomaterials in biological systems will be reflected by quantitative and qualitative differences in the pattern of proteins that bind to the nanomaterials surfaces. We propose to develop proteomics approaches to measure both the abundance and stoichiometry of nanomaterial-associated proteins, and mathematical models to predict protein binding properties of the nanomaterials based upon their surface physical and chemical properties. Our proposed approach will employ a dual quantitation strategy (18O labeling and label free) to measure differential binding of proteins in biofluid to nanomaterials. The accurate mass, liquid chromatography elution time and tandem mass spectrum obtained by the hybrid high mass resolution mass spectrometer will be used for accurate peptide/protein identification and quantitation. Quantitative structure activity relationship (QSAR) modeling will be employed to formalize the physical-chemical and surface properties of nanomaterials which determine the initial interaction of nanomaterials with proteins in biofluid.

Project Details

Start Date
2008-08-14
End Date
2011-08-21
Status
Closed

Team

Principal Investigator

Tao Liu
Institution
Pacific Northwest National Laboratory

Related Publications

Zhang H, KE Burnum, ML Luna, BO Petritis, JS Kim, W Qian, RJ Moore, A Heredia-Langner, BJM Webb-Robertson, BD Thrall, DG Camp, II, RD Smith, JG Pounds, and T Liu. 2011. "Quantitative proteomic analysis of adsorbed plasma proteins classifies nanoparticles with different surface properties and size." Proteomics 11(23):4569-4577. doi:10.1002/pmic.201100037