Biofilm Studies using Multi-modal Molecular Imaging Agents
EMSL Project ID
3180
Abstract
The Bornhop research group has developed a new class of multi-modal contrast agent. These unique molecular imaging agents exhibit bright luminescence, give magnetic resonance imaging (MRI) contrast, have long emission lifetimes for detection in the zero noise regime and can be tuned structurally for specific and sensitive molecular recognition. These polyazamacrocyclic lanthanide chelate complexes have been found to be non-toxic and useful for in-vitro diagnostics and in-vivo disease detection. Research proposed here will employ newly developed molecular imaging agents that will provide bi-modal imaging capabilities and allow the study of previously intractable biological problems. By preparing cocktails of both the Gd3+ and Eu3+ or Tb3+ complexes, it has been possible to obtain non-invasive anatomic-scale images by MRI and perform microscopic-scale imaging by fluorescence on the same cell. Under an expanded imaging effort and using the unique resources at the EMSL our new, targeted agents will be used to study biological function in microbes of interest to DOE.Facility access is requested in two separate time blocks:
? Four days of access early in the project period (Winter 2002-3) to two liquid-state NMR instruments (500 and 800 MHz) to measure the field and concentration-dependent MR spin-lattice (T1) and spin-spin (T2) relaxation times, and the lanthanide-induced chemical shift upon the 1H NMR of neighboring water molecules. This information will aid in the fine tuning of optimal contrast agents for subsequent microbial studies.
? Ten days of access late in the project period (Summer 2003) to EMSL?s combined optical/magnetic resonance microscope for integrated optical and magnetic resonance microscopy of bacterial consortia employing lanthanide chelate complexes.
Project Details
Project type
Capability Research
Start Date
2002-12-19
End Date
2004-01-12
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Fernandez CA, SK Nune, RK Motkuri, PK Thallapally, CM Wang, J Liu, GJ Exarhos, and BP McGrail. 2010. "Synthesis, characterization, and application of metal organic framework nanostructures." Langmuir 26(24):18591–18594. doi:10.1021/la103590t
Liu J, PK Thallapally, and DM Strachan. 2013. "Enhanced Nole Gas Adsorption in Ag@MOF-74Ni." Chemical Communications 50:466-468. doi:10.1039/C3CC47777K
Nune SK, PK Thallapally, A Dohnalkova, CM Wang, J Liu, and GJ Exarhos. 2010. "Synthesis and Properties of Nano Zeolitic Imidazolate Frameworks." Chemical Communications 46(27):4878–80.
Tian J, PK Thallapally, SJ Dalgarno, BP McGrail, and JL Atwood. 2009. "Amorphous Molecular Organic Solids for Gas Adsorption." Angewandte Chemie International Edition 48(30):5492 –5495.