Comparative study of protein profiles of Desulfovibrio desulfuricans G20 under different metabolism conditions
EMSL Project ID
34313
Abstract
Abstract: Desulfovibrio has very diversified metabolism styles. It can grow by fermentation using pyruvate, by respiration of organic substrates or hydrogen coupled to sulfate reduction or by syntrophic growth with methanogens. As an important electron carrier and intermediate in redox reaction, H2 is important for every type of metabolism. Desulfovibrio contains unique hydrogen (H2) metabolism pathways, which can produce H2 during the oxidation of organic substrated and also can oxidize H2 and use it co-metabolicaly during the degradation of organic compounds or as sole electron donor. Many proteins involved in above metabolic processes involving H2 production and oxidation, and energy generation pathways under different growth conditions have not been identified. The research proposed here will detect novel proteins possibly involved in these metabolic and energy generation pathways and to investigate metabolism netwoks of H2 production and oxidation in D. desulfuricans G20. For this proposal, we will compare the protein profiles of Desulfovibrio desulfuricans G20 under sulfodogenic (Lactate/sulfate), hydrogentrophic (H2/sulfate), syntrophy (with methanospirillum hungatei JF1 and Syntrophomonas Wolfei) and fermentation (pyruvate) growth. Proteins profiles will be compared to identify proteins that are specifically used for each pathway. Cytochrome c3 is a key electron transfer protein used during respiration and has been shown to be a critical component of the H2 oxidation pathway. We will use co-immunoprecipitation methodology and proteomic analysis to identify proteins interacting with cytochrome c3.
Project Details
Project type
Exploratory Research
Start Date
2009-11-19
End Date
2010-11-21
Status
Closed
Released Data Link
Team
Principal Investigator