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Proteomics of Morphology Determination in a Fungus


EMSL Project ID
3511

Abstract

The white rot fungus, Phanerochaete crysosporium, is a saprophytic organism isolated from decaying wood chips. It is one of few organisms that produce ligninase enzymes. These enzymes are required for lignin degredation. There are numerous reports on the cultivation of this filamentous fungus in stirred-tank bioreactors as free mycelia and immobilized on various support materials in an effort to develop a process for making ligninases. The filamentous nature of the fungus is problematic for stirred-tank bioprocessing.
In fungal bioprocesses. control of the filamentous growth habit is critical as has been observed in several commercial processes for making pharmaceuticals, organic acids, and enzymes. The control of morphology is likely to be a complex phenomenon relying on multiple differentially expressed proteins. This study employs the mass spectrometry and computational tools available within EMSL to anaylze differences in the proteome of a filamentous fungus in two distinct morphological states-mycelia and pellets.

Project Details

Project type
Exploratory Research
Start Date
2003-04-17
End Date
2005-01-11
Status
Closed

Team

Principal Investigator

Linda Lasure
Institution
Pacific Northwest National Laboratory

Team Members

Ellen Panisko
Institution
Pacific Northwest National Laboratory