Novel flow based biomolecule immobilization on nanoporous scaffolds
for enhanced biosensor performance and drug delivery applications
EMSL Project ID
39946
Abstract
Biosensors play indispensible roles in disease diagnosis, drug screening, and forensic applications. One important area of biosensor research is the immobilization of biomolecules (i.e. enzyme, DNA) with retained or enhanced activities as it is critical to enhance biosensor performance. Nanoporous scaffolds hold an enormous potential in improving the performance of biosensors as porous scaffolds can serve as excellent encapsulating matrix to various bioagents. The central objective of this PNNL proposal is to use the knowledge base gained recently by PI's group on synthesizing biocompatible and stable nanoporous scaffolds with microfluidics method, to develop a robust biomolecule immobilization procedure for enhanced biosensor performances and drug delivery applications. Specific research objectivesare: (1) Tuning the morphology of flow induced nanoporous scaffolds by varying chemical compositions, microfluidic design, and flow conditions, with both experiments, modeling and detailed nano-morphology studies; (2) Optimization of biomolecule immobilization in nanoporous scaffolds for sensor applications, including enzyme and DNA encapsulation with sensor performance tests.
This proposed research would lead to the discovery of novel scaffolds and biomolcule immobilization methods, while open up new pathways for designing simple, fast, biocompatible, and cost-effective process for enhanced sensor performance and on-site testing
of a variety of biomolecules.
Project Details
Project type
Limited Scope
Start Date
2010-09-01
End Date
2010-10-10
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members