Structure determination of membrane proteins
EMSL Project ID
3995
Abstract
Membrane proteins, although representing about one third of encoded proteins, are structurally undercharacterized. Yet, with the recent improvements in field and experiments, solution NMR spectroscopy becomes a promising techniques for assessing the structures of membrane proteins. Two systems of medical relevance are currently being studied in our lab as model membrane proteins, a region of the epidermal growth factor receptor, and an integral membrane protein from mycobacterium tuberculosis, and solution NMR methods are applied to obtain strucutral information in isotopric, micellar conditions.
Project Details
Project type
Capability Research
Start Date
2003-10-01
End Date
2004-10-07
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
3. S. A. Nizkorodov, J. Laskin, A. Laskin ?Molecular Chemistry of Organic Aerosols Through Applications of the High Resolution Mass Spectrometry? Phys. Chem. Chem. Phys., 13, 3612?3629, (2011). doi:10.1039/C0CP02032J (INVITED PERSPECTIVE ARTICLE)
Alexandrova AN, MJ Nayhouse, MT Huynh, JL Kuo, AV Melkonian, G Chavez, NM Hernando, MD Kowal, and CP Liu. 2012. "Selected AB?²-/- (A = C, Si, Ge; B = Al, Ga, In) Ions: a Battle Between Covalency and Aromaticity, and Prediction of Square Planar Si in SiIn?²-/-." Physical Chemistry Chemical Physics. PCCP 14:14815–14821. doi:10.1039/c2cp41821e
Bateman, AP. 2011. "Chemical Composition and Photochemical Evolution of Limonene Secondary Organic Aerosol Studied Using High Resolution Electrospray Ionization Mass Spectrometry" Dissertation, University of California, Irvine.
Bateman AP, J Laskin, A Laskin, and S Nizkorodov. 2012. "Applications of High-Resolution Electrospray Ionization Mass Spectrometry to Measurements of Average Oxygen to Carbon Ratios in Organic Aerosols." PNNL-SA-89371, Pacific Northwest National Laboratory, Richland, WA.
Bateman AP, S Nizkorodov, J Laskin, and A Laskin. 2010. "High-Resolution Electrospray Ionization Mass Spectrometry Analysis of Water- Soluble Organic Aerosols Collected with a Particle into Liquid Sampler ." Analytical Chemistry 82(19):8010-8016. doi:10.1021/ac1014386
Bateman AP, S Nizkorodov, J Laskin, and A Laskin. 2011. "Photochemical processing of secondary organic aerosols dissolved in cloud droplets." PNNL-SA-78898, Pacific Northwest National Laboratory, Richland, WA. [Unpublished]
Eckert PA, A Laskin, J Laskin, and PJ Roach. 2011. "Chemical Characterization of Crude Petroleum Using Nano-DESI Mass Spectrometry" Analytical Chemistry dx.doi.org/10.1021/ac202801g | Anal. Chem. 2012, 84, 1517?1525
Fooshee DR, TB Nguyen, SA Nizkorodov, J Laskin, A Laskin, and P Baldi. 2012. "COBRA: A Computational Brewing Application for Predicting the Molecular Composition of Organic Aerosols." Environmental Science & Technology 46(11):6048-6055. doi:10.1021/es3003734
Ilton ES, and PS Bagus. 2011. "XPS Determination of Uranium Oxidations States." Surface and Interface Analysis 43(13):1549-1560. doi:10.1002/sia.3836
Ilton ES, JS Lazama Pacheco, JR Bargar, Z Shi, J Liu, L Kovarik, MH Engelhard, and AR Felmy. 2012. "Reduction of U(VI) Incorporated in the Structure of Hematite." PNNL-SA-87404, Pacific Northwest National Laboratory, Richland, WA.
Laskin J, A Laskin, and S Nizkorodov. 2013. "New Mass Spectrometry Techniques for Studying Physical Chemistry of Atmospheric Heterogeneous Processes." International Reviews in Physical Chemistry 32(1):128-170. doi:10.1080/0144235X.2012.752904
Laskin J, PA Eckert, PJ Roach, BS Heath, SA Nizkorodov, and A Laskin. 2012. "Chemical Analysis of Complex Organic Mixtures Using Reactive Nanospray Desorption Electrospray Ionization Mass Spectrometry." Analytical Chemistry 84(16):7179-7187. doi:10.1021/ac301533z
Lee HJ, A Laskin, J Laskin, and SA Nizkorodov. 2013. " Excitation-emission spectra and fluorescence quantum yields for fresh and aged biogenic secondary organic aerosols." Environmental Science & Technology 47(11):5763-5770. doi:10.1021/es400644c
Massey MS, JS Lezama-Pacheco, ME Jones, ES Ilton, JM Cerrato, JR Bargar, and S Fendorf. "Competing retention pathways of uranium upon reaction with Fe(II)." Geochimica et Cosmochimica Acta. doi:10.1016/j.gca.2014.07.016
Nguyen TB, A Laskin, J Laskin, and S Nizkorodov. 2012. "Direct Aqueous Photochemistry of Isoprene High-NOx Secondary Organic Aerosol." Physical Chemistry Chemical Physics. PCCP 14(27):9702-9714. doi:10.1039/c2cp40944e
Nguyen TB, A Laskin, J Laskin, and S Nizkorodov. 2013. "Brown carbon formation from ketoaldehydes of biogenic monoterpenes." Faraday Discussions 165:473-494. doi:10.1039/c3fd00036b.
Nguyen TB, J Laskin, A Laskin, and S Nizkorodov. 2011. "Nitrogen Containing Organic Compounds and Oligomers in Secondary Organic Aerosol Formed by Photooxidation of Isoprene." Environmental Science & Technology 45(16):6908-6918. doi:10.1021/es201611n
Nguyen TB, PB Lee, KM Updyke, DL Bones, J Laskin, A Laskin, and S Nizkorodov. 2012. "Formation of Nitrogen- and Sulfur-Containing Light-Absorbing Compounds Accelerated by Evaporation of Water from Secondary Organic Aerosols." Journal of Geophysical Research. D. (Atmospheres) 117:D01207. doi:10.1029/2011JD016944
Nguyen TB, PJ Roach, J Laskin, A Laskin, and S Nizkorodov. 2011. "Effect of Humidity on the Composition and Yield of Isoprene Photooxidation Secondary Organic Aerosol." PNNL-SA-78897, Pacific Northwest National Laboratory, Richland, WA. [Unpublished]
Nguyen TB, S Nizkorodov, A Laskin, and J Laskin. 2013. "An approach toward quantification of organic compounds in complex environmental samples using high-resolution electrospray ionization mass spectrometry." Analytical Methods 5(1):72-80. doi:10.1039/c2ay25682g
O'Brien RE, TB Nguyen, A Laskin, J Laskin, PL Hayes, S Liu, JL Jimenez, LM Russell, S Nizkorodov, and AH Goldstein. 2013. "Probing Molecular Associations of Field-Collected and Laboratory-Generated SOA with Nano-DESI High-Resolution Mass Spectrometry." Journal of Geophysical Research. D. (Atmospheres) 118(2):1042-1051.
Sebastien Kerisit, Andrew R. Felmy, and Eugene S. Ilton (2011)
Atomistic Simulations of Uranium Incorporation into Iron (Hydr)Oxides. Environ. Sci. Tech., 45, 2770-2776
Singer DM, SME Chatman, ES Ilton, KM Rosso, JF Banfield, and G Waychunas. 2012. "Identification of simultaneous U(VI) sorption complexes and U(IV) nanoprecipitates on the magnetite (111) surface." Environmental Science & Technology 46(7):3811-3820. doi:10.1021/es203877x
Singer DM, SME Chatman, ES Ilton, KM Rosso, JF Banfield, and G Waychunas. 2012. "U(VI) sorption and reduction kinetics on the magnetite (111) surface." Environmental Science & Technology 46(7):3821-3830. doi:10.1021/es203878c
Skomurski FN, ES Ilton, MH Engelhard, BW Arey, and KM Rosso. 2011. "Heterogeneous Reduction of U6+ by Structural Fe2+ From Theory and Experiment." Geochimica et Cosmochimica Acta 75(22):7277-7290. doi:10.1016/j.gca.2011.08.006
Zhang J, and AN Alexandrova. 2011. "Structure, Stability, and Mobility of Small Pd Clusters on the Stoichiometric and Defective TiO2 (110) Surfaces." Journal of Chemical Physics 135:174702. doi:10.1063/1.3657833
Zhang J, and AN Alexandrova. 2012. "Double ?-Aromaticity in a Surface-Deposited Cluster: Pd4 on TiO2 (110)." The Journal of Physical Chemistry Letters 3(6):751-754. doi:10.1021/jz300158s
Zhang J, and AN Alexandrova. 2013. "The Golden Crown: A Single Au Atom that Boosts the CO Oxidation Catalyzed by a Palladium Cluster on Titania Surfaces." Journal of Physical Chemistry Letters 4(14):2250–2255. doi:10.1021/jz400981a
Zhang J, AP Sergeeva, M Sparta, and AN Alexandrova. 2012. "B13+: Photodriven Molecular Wankel Engine." Angewandte Chemie International Edition 51(34):8512–8515,. doi:10.1002/anie.201202674