Proteomics of secreted proteins and method of lignocellulose degradation in the Basidiomycete model system Schizophyllum commune
EMSL Project ID
47591
Abstract
An understanding of microbial wood breakdown is critical for designing highly efficient enzymatic cocktails required for lignocellulolytic biomass degradation. Fungi, in particular the white and brown rot fungi (phylum Basidiomycetes), are notably good at breaking down the tough fibers present in wood. To date, over 40 genomes of wood-decaying fungi have been or are currently being sequenced. Computational analysis of their genomes suggests a large number of genes predicted to be involved in plant lignocellulose degradation. However, their exact roles and the regulatory network behind wood decay are largely unknown, due to a lack of an appropriate genetic model system. Although individual genes have been expressed and studied in Ascomycetes and prokaryotes, these model systems are insufficient to study the whole process of wood decay due to their different lifestyle and long evolutionary distance from the wood decaying Basidiomycetes. Schizophyllum commune is a highly genetically tractable Basidiomycete wood decayer. Here we propose to study the enzymes secreted into the growth medium by S. commune during growth on wood and wood components. Furthermore we propose to study the effect these enzymes have on the wood and wood components.
Project Details
Project type
Large-Scale EMSL Research
Start Date
2012-10-01
End Date
2014-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Co-Investigator(s)