Single-Cell Chemical Imaging in Micro- and Nanofluidics for Biofuel Research
EMSL Project ID
47768
Abstract
Microorganisms have been heavily employed to synthesize practical chemical substances, a capability that that has been recently enhanced, or diversified via genetic engineering. To this end, biofuel synthesis from biomass (e.g., cellulose) has also attracted substantial interest recently as a renewable energy source. In this project, single-cell chemical imaging in microfluidics will be explored, aiming at monitoring metabolic kinetics, as well as identifying traits and factors associated with enhanced conversion efficiencies. The methodology involves the isolation and trapping of single cells in microfluidics and the chemical imaging the relevant synthetic processes over time. Imaging will be based both on fluorescence, as well as non-linear optical methods. By analyzing such microorganisms individually at the single cell level, the metabolic kinetics and cell-to-cell variation, along with desirable traits will be measured and further analyzed.
Project Details
Start Date
2013-02-11
End Date
2014-02-23
Status
Closed
Released Data Link
Team
Principal Investigator
Related Publications
Exploiting Bioprocessing Fluctuations to Elicit the Mechanistics of De Novo Lipogenesis in Yarrowia lipolytica
Vasdekis A, AM Silverman, and G Stephanopoulos. 2017. "Exploiting Bioprocessing Fluctuations to Elicit the Mechanistics of De Novo Lipogenesis in Yarrowia lipolytica." PLoS One 12(1):Online. doi:10.1371/journal.pone.0168889
Single microbe trap and release in sub-microfluidics
Vasdekis AE. 2013. "Single microbe trap and release in sub-microfluidics." RSC Advances 3(18):6343-6346. doi:10.1039/C3RA40369F
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