Temporal and Spatial Dynamics of Fungal Defense in Arabidopsis
EMSL Project ID
48690
Abstract
We seek a rapid access grant to support an upcoming NSF proposal (November, 2014). EMSL technical capabilities are essential to the feasibility and success of the proposed work. Our goal is to understand how both temporal and spatial regulation affect the relative resistance of plant tissues to fungal infection. We seek to reveal the differential accumulation of metabolites over time and across infected leaf tissue. In addition, to understand the role of jasmonate hormone production regulation, which is critical for plant resistance, we will image fluorescence markers to assess whether there is differential spatial capacity for jasmonate production upon infection. The abilities of EMSL facilities to provide coincident metabolomics analyses and spatial imaging combined with subcellular-level fluorescence detection to reflect jasmonate synthesis are necessary to gain a full dynamic examination of the complexities of cellular and chemical interactions that result when fungal infection confronts plant defense. The proposed work will improve our understanding of mechanisms and dynamics of plant defense and resistance by adding both temporal and spatial resolution to the active infection process. Such new knowledge may provide important critical insights for optimizing plant growth and survival, both for high biomass production and/or to improve carbon sequestration and water efficiency use in stressful environments.
Project Details
Project type
Limited Scope
Start Date
2015-01-15
End Date
2015-03-17
Status
Closed
Released Data Link
Team
Principal Investigator