Plant Biotic Defense Dynamics
EMSL Project ID
48766
Abstract
Plant survival strategies include balancing resource allocation to growth versus defense against biotic stress. Despite the importance of this balance for plant productivity, measured as biomass and metabolite output and/or reproductive fitness, the underlying regulatory principles controlling shifts in this balance remain largely unknown. The proposed work will elucidate molecular and metabolic profiles associated with both response to and recovery from fungal attack in Arabidopsis, with a focus on jasmonate-mediated defense. Foundational work with Arabidopsis as a model system can then be translated to species critical for biomass production and carbon cycling. Temporal dynamics of plant outputs, including circadian-controlled differential behaviors and the transient diversion of resources to defense pathways, will be revealed through metabolome, proteome and transcriptome profiling, as well as in situ metabolomics to provide the power of spatial resolution. Complementary and concurrent high resolution and quantitative imaging will reveal organelle dynamics, with a particular focus on peroxisomes, the site of the jasmonate phytohormone biosynthesis. The high throughput global and in situ analysis capabilities and imaging expertise of EMSL are critical for completion of this work. The diverse genetic resources available for Arabidopsis will facilitate the future verification of functional relevance of the system-wide component profiles, together providing new insights into fundamental understanding of how plants evolved to survive biotic environmental stress. Successful plant survival in the presence of biotic stress, such as fungal infection, is essential for the BER mission because of the central role plants play in both carbon and water cycling and because plants are rich sources for new bio-based fuels and products. The proposed work fits the EMSL Biosystem Dynamics and Design Science Theme, with the goal of uncovering the regulation of spatial and temporal parameters of metabolic processes critical for plant-fungal interaction and including the focus on peroxisome dynamics, which may play a central role in balancing plant defense versus growth and development. Thus, this work lays the foundation for building predictive models of plant biotic stress molecular and metabolic pathways, across the scales of subcellular organelles to host-pathogen interaction.
Project Details
Project type
Large-Scale EMSL Research
Start Date
2015-10-01
End Date
2018-03-31
Status
Closed
Released Data Link
Team
Principal Investigator
Related Publications
Dai Y., H.A. Ogilvie, Y. Liu, M. Huang, L. Markillie, H.D. Mitchell, and E.J. Borrego, et al. 2019. "Metadata of the article that will be visualized in OnlineFirst." Planta 250, no. 6. PNNL-SA-147546. doi:10.1007/s00425-019-03273-5