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Microbial Interactions with Natural Organic Matter from Oak Ridge FRC Sites


EMSL Project ID
49432

Abstract

Natural organic matter (NOM) is central to microbial food webs; and microbial activities metabolize and transform NOM, which in turn shapes the microbial community structure and function in the environment. This interaction between microbes and NOM is essential for understanding the carbon cycling in ecosystem. So far, little is known about the interplay between the physical and chemical characteristics of NOM and its turnover by microbial communities. In this study, our objectives include: 1. Identification and characterization of the natural NOM that is bioavailable for native microbes in FRC groundwater; 2. Correlate bioavailable carbon/energy source in FRC sediments to microbial community structure; 3. Provide a context for interpretation of carbon related gene-expression results. This research will fill critical knowledge gap on the metabolic potential of FRC microbial community especially with regards to NOM, and will improve our understandings on microbial activities on carbon cycling in ecosystem. The FT-ICR at EMSL may provide information for characterization of NOM at a molecular level, which is essential for studying the metabolism of NOM by microbial community.

Project Details

Project type
Limited Scope
Start Date
2016-04-01
End Date
2016-06-01
Status
Closed

Team

Principal Investigator

Romy Chakraborty
Institution
Lawrence Berkeley National Laboratory

Team Members

Xiaoqin Wu
Institution
Lawrence Berkeley National Laboratory

Related Publications

Wu X., L. Wu, P. Zhang, Q. Li, J. Zhou, N.J. Hess, and T. Hazen, et al. 2018. "Microbial Interactions With Dissolved Organic Matter Drive Carbon Dynamics and Community Succession." Frontiers in Microbiology 9. PNNL-SA-135810. doi:10.3389/fmicb.2018.01234