Morphology and Protein Profile of Bacterium-Membrane-Formed Nanovesicles
EMSL Project ID
49684
Abstract
The major goal of this proposal is to access the advanced facility at EMSL to obtain the preliminary data to support our grant application. The structure-activity relationship of nanomaterials is critical in many applications, such as energy security, environment and biology. The specific goal of proposed research is to request the EMSL facility (cryo-TEM and proteomics mass spectroscopy) to support us to dissect how the structure and protein composition of a new type of nanovesicles directly made from bacterial membrane contribute to their biological functions. The preliminary data show that we have successfully generated nanovesicles directly formed from the plasma membrane of eukaryotic and prokaryotic cells, but the detail of structure-function relationships of these nanovesicles are unknown. Here we propose a novel approach to generate bacterium-membrane-formed nanovsicles. Our hypothesis is that bacterium-membrane-formed nanovesicles composed of a wide range of membrane antigens from bacteria would boost immune systems to prevent bacterial invasion from lungs. The EMSL facility will provide a wide range of resources (such as cryo-TEM and proteomics mass spectroscopy) for us to determine the structure and protein profile of this new type of nanovesicles. We believe that the preliminary data would strongly support our grant application. If we are successfully funded, we could become a long-term user to support the EMSL missions in molecular-level discoveries in energy security, environment and biology.
Project Details
Project type
Limited Scope
Start Date
2016-12-14
End Date
2017-02-13
Status
Closed
Released Data Link
Team
Principal Investigator