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GoBrachy: Developing a Metabolite-Trait Association Network Model for Carbon Allocation in Brachypodium


EMSL Project ID
49714

Abstract

Aboveground biomass (AGBM) and belowground biomass (BGBM) of Brachypodium lines will be harvested at week seven and parallel lines will be allowed to grow to senescence (B. distachyon) or longer (B. sylvaticum and B. pinnatum) for measurement of AGBM and BGBM. High-coverage and multi-platform metabolomics will be performed on AGBM and BGBM. Multi-platform metabolite profiles will be summarized to obtain non-redundant data and regression analyses will be employed to remove platform-specific bias and noise . Trait data will be pretreated to remove correlation with genetic population structure and used together with metabolite profiles to generate a Metabolite Trait Association Network. Cross validation and independent experiments will be used to validate the derived model. Metabolite profiles will be used to build a Brachypodium Metabolite Database.

Project Details

Start Date
2017-02-17
End Date
2017-09-30
Status
Closed

Team

Principal Investigator

Christer Jansson
Institution
Environmental Molecular Sciences Laboratory

Team Members

Pubudu Handakumbura
Institution
Environmental Molecular Sciences Laboratory

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