Enhanced Isotope Ratio Performance: Liquid Sampling-Atmospheric Pressure Glow Discharge (LS-APGD) Ionization Source/Orbitrap Coupling
EMSL Project ID
50366
Abstract
Collaborative efforts over the last 6 years between scientists at Clemson University and EMSL have demonstrated a very positive efficacy in the coupling of a highly diverse ionization source, the liquid sampling-atmospheric pressure glow discharge (LS-APGD) microplasma and a conventional ThermoScientific Orbitrap mass analyzer. In particular, the microplasma ionization source provides a means for elemental analysis of liquids, solids, and gases, enabling facile environmental and biological characterization. Coupling with the Orbitrap mass spectrometer provides exceedingly high mass resolution (m/delta-m approx. 100,000), high sensitivity (<0.01 ng), and to much surprise isotope ratio performance that rivals many more standard analytical systems. In addition to the publication of 7 peer-reviewed manuscripts, over 20 conference presentations, one granted US patent, and one pending patent have resulted. Over the last few months, a collaborative effort between the Clemson University Research Foundation (CURF) and the Pacific Northwest National Laboratory Technology Innovation Program has been focused on increasing the features of the LS-APGD/Orbitrap system. The target of these efforts was to consolidate knowledge through the previous experiences, the sequential improvement in source performance, and implementation of designed for purpose hardware to affect a transportable system that can be interfaced to any of the multiple Orbitrap platforms on the market. At present, the electrospray ionization (ESI) sources most commonly applied in proteomics analysis, and come standard with Orbitrap instruments, can be replaced by the microplasma source in less than one minute. In doing so, elemental analyses can be applied across many different fields of analytical chemistry, biological chemistry, environmental chemistry, and geochemistry, to yield spectra with mass resolution never before obtained on currently available instrumentation. Such capabilities hold the possibility for tremendous impact.
Studies are proposed here to integrate the ion source hardware developed at Clemson University with an integrated control system designed and developed at EMSL/PNNL. The combination of the new ion source assembly and control unit are expected to result in an easily adoptable and flexible system. We propose here the use of a ThermoScientific Exactive Orbitrap instrument housed in the EMSL facility (specifically the system located in EMSL 1310 laboratory). In fact, the specific instrument employed in the initial efforts of this coupling will be employed. Extensive performance data exist for the "component-based" LS-APGD ionization source on this instrument, including analytical response functions, spectral composition, sample-to-sample reproducibility, and isotope ratio precision. It is anticipated that complete integration will provide improvements on all fronts.
Rapid Access is requested at this point as funds have already been secured for the PI and his graduate students to attend the Institute for Nuclear Materials Management (INMM) workshop taking place in Richland, WA during the first week of May. In addition, deadlines are rapidly approaching for submission of the work to a special issue of a journal dedicated to novel instrumentation development. Actual on-site work will be limited to 4 days of work during the week of April 30.
Project Details
Project type
Limited Scope
Start Date
2018-04-30
End Date
2018-06-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Hoegg E.D., S. Godin, J. Szpunar, R. Lobinski, D.W. Koppenaal, and R.K. Marcus. 2019. "Ultra-High Resolution Elemental/Isotopic Mass Spectrometry (m/?m> 1,000,000): Coupling of the Liquid Sampling-Atmospheric Pressure Glow Discharge with an Orbitrap Mass Spectrometer for Applications in Biological Chemistry and Environmental Analysis." Journal of the American Society for Mass Spectrometry 30, no. 7:1163-1168. PNNL-SA-140817. doi:10.1007/s13361-019-02183-w
Hoegg E.D., S. Godin, J. Szpunar, R. Lobinski, D.W. Koppenaal, and R. Marcus. 2019. "Coupling of an atmospheric pressure microplasma ionization source with an Orbitrap Fusion Lumos Tribrid 1M mass analyzer for ultra-high resolution isotopic analysis of uranium." Journal of Analytical Atomic Spectrometry 34, no. 7:1387-1395. PNNL-SA-142442. doi:10.1039/C9JA00154A