Reverse Engineering CAZyme Hypersecretion in Filamentous Fungi
EMSL Project ID
50413
Abstract
In this project, we are aiming to uncover the basis of carbohydrate active enzyme (CAZyme) hypersecretion in the Neurospora crassa ?frp-1 mutant. We also want to reverse engineer this phenotype into the industrially relevant fungus Myceliophthora thermophila. Investigation of the intracellular proteome of N. crassa wild type (WT) and ?frp-1 will be instrumental for this endeavor, since the FRP-1 protein is a member of the F-box protein family and therefore likely involved in degradation of unknown intracellular protein targets, which participate in the induction of CAZyme-encoding genes, leading to hypersecretion in the mutant strain. The proposed comparative proteomic study will employ an early and later time point of proteome analysis in WT and ?frp-1 to uncover primary target proteins and secondary proteome changes in response to frp-1 deletion. This will not only lead to an improved mechanistic understanding of the frp-1 deletion on CAZyme hypersecretion in N. crassa and M. thermophila, but also help to expand the knowledge of the regulation of CAZymes, sugar transporters and related metabolisms in filamentous fungi to design improved fungal strain engineering strategies for enzyme production. Project Details
Project type
Limited Scope
Start Date
2018-10-16
End Date
2018-12-16
Status
Closed
Released Data Link
Team
Principal Investigator