Multiscale analysis to explore the molecular landscape of Sorghum bicolor stem over time and space
EMSL Project ID
51398
Abstract
Sorghum bicolor is an important bioenergy crop because it has high nutrient and water use efficiency, and produces a large quantity of biomass that can be processed into ethanol for biofuels. Many genomic resources exist for sorghum, and the transformation efficiency of different sorghum lines is increasing. Thus, sorghum is an ideal crop to research for improved biofuel and bioproduct production. One potential advancement would be to engineer sorghum stems to accumulate bioproducts, which would decrease the burden on conversion groups in the biofuel industry. Despite the wealth of sorghum sequencing data for gene expression and genetic variation, there is little information available about sorghum stem biology. Likewise, no studies have focused on single cell or single cell-type specific molecular biology in sorghum stems. A high-resolution temporal and spatial transcriptome, proteome, and metabolome of sorghum stem cell-types would provide unprecedented insight into differential expression and regulation of genes and gene products. An improved understanding of tissue and cell-type gene networks will accelerate the identification of gene targets for engineering for crop improvement, including the potential identification of promoter elements that contribute to stem cell-type specific expression. Recent advances in laser capture microdissection and RNA-sequencing have made single cell and single cell-type "omic" studies feasible. The purpose of this proposal is to use a protocol, recently developed by EMSL and BRC researchers, for stem cell-type RNA-sequencing to explore the spatial and temporal transcriptome of sorghum stem cell-types, and expand this analysis to include the proteome and metabolome. Specifically, four cell types over two developmental stages. This proposal is mutually beneficial to BRC and EMSL researchers by increasing our collective expertise in molecular and biochemical approaches for sorghum, an important bioenergy crop. EMSL has a unique combination of expertise and instrumentation for method development and testing, which is not available at our academic institutions. The proposed project will provide a molecular atlas of sorghum stems over development. The results of this exploratory proposal will be an important and impactful resource for CABBI BRC and GLBRC.
Project Details
Project type
Large-Scale EMSL Research
Start Date
2020-10-01
End Date
2022-12-31
Status
Closed
Released Data Link
Team
Principal Investigator
Co-Investigator(s)
Team Members