High throughput generation of small molecule probes for discovery of unique cellular functions using Substrate Activity Screening
EMSL Project ID
51699
Abstract
We propose a method to demystify the massive catalog of proteins-of-unknown-function accumulated by recent advances in genome sequencing by direct assignment of function. We will query enzyme activity using large libraries of non-natural fluorogenic substrates to create an easy, high throughput means of screening proteins for unique functions. This project supports the EMSD/EMSL goal of establishment of a Digital Phenome through providing a method to create rich functional profiling data. The simplicity of the solid-phase synthesis approach will allow EMSL to create large, customizable, small molecule probe libraries with undergraduate-level chemical synthetic expertise.
Project Details
Start Date
2020-10-26
End Date
2022-11-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members