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Erin Bredeweg

Institution
Environmental Molecular Sciences Laboratory

Projects

Development of chemical probes for live cell Raman imaging

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Sankarganesh Krishnamoorthy
Biological molecular phenotyping requires characterization tools that can record multiple biological processes and molecular transformations simultaneously in real time. Currently, fluorescence…

Mapping microbial transporters with small molecule fluorescent probes

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Erin Bredeweg
Objectives: There is a gap in our ability to measure transport of cell materials (sugars, lipids, amino acids, metabolites, cofactors, etc.) across membranes, and to observe transporter localization…

Decreasing the amount of material required for gross biological carbon and nitrogen flux measurements by more than 12 orders of magnitude using ion implantation isotope dilution and stable isotope measurements by NanoSIMS

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
John Cliff
Advances in mass spectrometry coupled to stable isotope probing techniques have profoundly influenced our understanding of process level biology and biogeochemistry. When stable isotope compounds and…

Yarrowia lipolytica for biotechnology and synthetic biology

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Scott Baker
The team at PNNL is using a combination of flow cytometry, microscopy and lipidomics to establish baseline cell biological and metabolite data on lipid production by the W29 strain of Yarrowia…

Yarrowia lipolytica for biotechnology and synthetic biology

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Erin Bredeweg
The team at PNNL is using a combination of flow cytometry, microscopy and lipidomics/metabolomics to establish baseline cell biological and metabolite data on DNA agility in eukaryotic organisms, and…