Functional and structural analysis of microbial expansin-related proteins that transform lignocellulosic biomass
EMSL Project ID
60027
Abstract
This project brings together functional genomics, structural biology, and advanced techniques in material science, to evaluate the untapped potential of microbial expansin-related proteins in the production of bio-based chemicals and materials. In particular, the proposed project will leverage earlier bioinformatics analyses, as well as gene synthesis efforts completed at the JGI, to investigate the impact of microbial expansin-related proteins on lignocellulose organization, including cellulose microfibril structure, matrix copolymer assembly, and the porosity of the cell walls. The experimental approach includes in-situ Biological Small Angle Neutron Scattering (Bio-SANS) measurements to follow the protein-induced structural changes in lignocellulosic biomass in real-time. The microbial expansin-related proteins selected for the study will include examples from different phylogenetic groups, which will permit structure-functional studies of protein targets that modify lignocellulose organization. Herein, the solution structures will first be obtained using SAX, which will guide protein selection and preparation for detailed structural analysis using solution NMR. Accordingly, the proposed project directly aligns with FICUS Research concerning Biofuels, Biomaterials, and Bioproducts, and combines EMSL capabilities in structural biology with CSMB capabilities at Oak Ridge National Laboratory in Bio-SANS. Moreover, the project PI and co-PI possess considerable experience in leading national and international research initiatives, which will ensure the successful completion of project goals.
Project Details
Project type
FICUS Research
Start Date
2021-10-01
End Date
2023-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Co-Investigator(s)