Skip to main content

Proteomic Analysis of Deinococcus radiodurans Under Oxidative StressIrradiation


EMSL Project ID
6190

Abstract

The bacterium Deinococcus radiodurans is able to survive extremely high doses of gamma-radiation and we have recently shown that high intracellular concentrations of Mn(II) facilitate resistance by limiting oxidative stress during recovery. This work will utilize proteomic instrumentation at PNNL and gamma-irradiation facilities at USUHS. The experimental goals include determining the effect of oxygen and Mn(II) concentrations on the global set of D. radiodurans proteins and a sub-fraction of Mn-dependent proteins associated with the D. radiodurans genome. Collectively, this work will facilitate understanding the relationship between energy metabolism and radiation toxicity.

Project Details

Project type
Exploratory Research
Start Date
2004-01-09
End Date
2007-01-14
Status
Closed

Team

Principal Investigator

Michael Daly
Institution
Uniformed Services University of the Health Sciences

Related Publications

Appel AM, JE Bercaw, AB Bocarsly, H Dobbek, DL DuBois, M Dupuis, JG Ferry, E Fujita, R Hille, P Kenis, CA Kerfeld, RH Morris, CHF Peden, A Portis, S Ragsdale, TB Rauchfuss, J Reek, LC Seefeldt, RK Thauer, and GL Waldrop. 2013. "Frontiers, Opportunities, and Challenges in Biochemical and Chemical Catalysis of CO2." Chemical Reviews 113(8):6621?6658. doi:10.1021/cr300463y