Proteomic Analysis of Deinococcus radiodurans Under Oxidative StressIrradiation
EMSL Project ID
6190
Abstract
The bacterium Deinococcus radiodurans is able to survive extremely high doses of gamma-radiation and we have recently shown that high intracellular concentrations of Mn(II) facilitate resistance by limiting oxidative stress during recovery. This work will utilize proteomic instrumentation at PNNL and gamma-irradiation facilities at USUHS. The experimental goals include determining the effect of oxygen and Mn(II) concentrations on the global set of D. radiodurans proteins and a sub-fraction of Mn-dependent proteins associated with the D. radiodurans genome. Collectively, this work will facilitate understanding the relationship between energy metabolism and radiation toxicity.
Project Details
Project type
Exploratory Research
Start Date
2004-01-09
End Date
2007-01-14
Status
Closed
Released Data Link
Team
Principal Investigator
Related Publications
Appel AM, JE Bercaw, AB Bocarsly, H Dobbek, DL DuBois, M Dupuis, JG Ferry, E Fujita, R Hille, P Kenis, CA Kerfeld, RH Morris, CHF Peden, A Portis, S Ragsdale, TB Rauchfuss, J Reek, LC Seefeldt, RK Thauer, and GL Waldrop. 2013. "Frontiers, Opportunities, and Challenges in Biochemical and Chemical Catalysis of CO2." Chemical Reviews 113(8):6621?6658. doi:10.1021/cr300463y