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Combined transcriptome and proteome analysis of Shewanella oneidensis MR-1 etrA and arcA mutant strains


EMSL Project ID
8594

Abstract

Shewanella oneidensis etrA and arcA deletion strains and the parental strain were each grown in continuous culture for 410 hours. The growth conditions were altered from an aerobic steady state, to a microoxic steady state and to an anaerobic steady state to examine the contribution of each regulator in S. oneidensis. Samples were collected at each steady state for organic acid, proteome, cytochrome and transcriptome analyses. Samples were also harvested at 0, 5, 10, 20, 30, 40, 50, 60, 90, 120, and 150 minutes after transition from aerobic to microoxic steady states for transcriptome studies.

Project Details

Project type
Exploratory Research
Start Date
2004-04-22
End Date
2006-06-08
Status
Closed

Team

Principal Investigator

Steven Brown
Institution
Oak Ridge National Laboratory

Team Members

Joe (jizhong) Zhou
Institution
Stephenson Research and Technology Center

Yuri Gorby
Institution
J. Craig Venter Institute

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