Arabidopsis thaliana
EMSL Project ID
9101
Abstract
Arabidopsis is a full sequenced organism which is favored used for plant research. Since the vascular bundle is an organ of vitally importance in plants, not only for the transport of water and metabolites but also for the transport of signal substances and proteins, I want to identify proteins of that tissue. There is only little known about the proteins present in the vascular bundle in particular about those which are transported in the phloem and xylem. The identification of the proteins in the vascular bundle will give more insight into the things happening in the plant. Further on the vascular bundle seems to play a big role in stress response (e.g. proteins of phloem sap of cucumber show a very different pattern in 2DE in drought stress experiments). That is why a want to investigate the influence of a pathogen infection (powdery mildew ? infects only the leaves of the plants) to the composition of the proteins in the vascular bundles.
Since it is difficult to get samples of vascular bundles of the intact plant, I use a method which is new in plant research and allows sampling even of single cells without contamination of surrounding tissue. Cross sections from stems are made using a cryostat, are dried and used for Laser Microdissection/Laser Pressure Catapulting. Applying this technique a defocused laser beam catapults the tissue of interest into a cap which is placed above the slide. As you might assume the amount of sample you get is limited. Since the small sample amount doesn?t allow extended protein analysis using conventional approaches (2D gel electrophoresis, tryptic digestion, MS/MS) an alternative more sensitive method is desirable. The application of the LCQ and the FTICR is a great possibility to achieve best results for my research interests.
Project Details
Project type
Exploratory Research
Start Date
2004-05-13
End Date
2005-03-28
Status
Closed
Released Data Link
Team
Principal Investigator