Brian Lower

Quorum Sensing in a Dissimilatory Metal Reducing Bacterium

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Michael Hochella

Project type

Large-Scale EMSL Research

Quorum sensing is a versatile regulatory mechanism exhibited by several members of Bacteria. Even though this area of research is a rapidly growing field with clear environmental applications, it…

Development of an integrated atomic force / fluorescence microscope at EMSL

Lead Institution

The Ohio State University

Principal Investigator

Brian Lower

Project type

Large-Scale EMSL Research

We propose to develop a new spectroscopic/microscopic capability at EMSL that combines atomic force microscopy (AFM) with fluorescence microscopy allowing for simultaneous force and fluorescence…

Specificity of Shewanella oneidensis outer membrane cytochromes
OmcA and OmcB towards iron- and manganese-oxide surfaces

Lead Institution

The Ohio State University

Principal Investigator

Brian Lower

Project type

Large-Scale EMSL Research

Metal-reducing bacteria have a unique ability to reduce solid-phase iron- and manganese- oxides in anaerobic environments. Unlike oxygen, Fe and Mn oxides, in a solid form, cannot diffuse across the…

Basis of Microbial Membrane Attachment to Mineral Surfaces
and Ionic Contaminants Uptake

Lead Institution

The Ohio State University

Principal Investigator

Brian Lower

Project type

Large-Scale EMSL Research

Allocation of resources at EMSL facilities, both experimental and computational, is sought for the extension of our current DOE-funded biogeochemistry project. The project focus lies on the molecular…

Capture and Reduction of Metal Ions out of the
Environment by Biomolecular Systems

Lead Institution

Universidade Federal de Pernambuco

Principal Investigator

Roberto Lins Neto

Project type

Grand Challenge

The use of microbes and/or microbial components has been proven to be an efficient strategy for in situ environmental remediation technologies. The requested allocation of computer time is for…

Development of Single Molecule Force Microscopy Capability to Examine the Moleuclar Mechanism of Lignocellulosic Material Degradation

Lead Institution

The Ohio State University

Principal Investigator

Brian Lower

Project type

Large-Scale EMSL Research

The objective of this proposal is to develop a novel capability based on scanning probe microscopy to examine the enzyme-catalyzed hydrolysis of insoluble lignocellulosic material at the molecular…

Microscale and spectroscopic investigations in geochemical and microbiological systems

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Zachara

Project type

Large-Scale EMSL Research

Apply experimental manipulation and field investigations to understanding the behavior of selected chemical and microbial components in natural systems. The research is targeted towards compounds…

Geochemical Interactions of Fe-Sulfide Minerals with Uranium and Microorganisms
in Sediments from the DOE UMTRA site at Rifle, Colorado

Lead Institution

The Ohio State University

Principal Investigator

Brian Lower

Project type

Large-Scale EMSL Research

Previous biostimulation field-experimental studies (by injecting acetate into the aquifer)conducted at the DOE UMTRA (Uranium Mill Tailings Remedial Action) site in Rifle, CO, have demonstrated that…

Computer Simulations of Protein-Nanomaterial Interactions

Lead Institution

Universidade Federal de Pernambuco

Principal Investigator

Roberto Lins Neto

Project type

Capability Research

The development of nanotechnology has led to an increasing interest in interfacing biological molecules with nanomaterials. Proteins have been used to functionalize nanomaterials controlling their…

Coupling of Fe and Tc Speciation in Subsurface Sediments: Implications to Long-Term Tc Immobilization (Zachara NABIR-Fe/Tc, PNNL Scope # 40761)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Zachara

Project type

Exploratory Research

Creating an in-situ bio-barrier containing sediment-bound, biogenic FE(II) to reduce and collect mobile Tc(VII)O4 - The research objective is to provide scientific insights on the rates of reductive…

Computational Simulations of Antimicrobial Polymeric Surfaces and Biological Membranes

Lead Institution

Universidade Federal de Pernambuco

Principal Investigator

Thereza Soares Da Silva

Project type

Exploratory Research

Medical devices can give rise to treatment-resistant infections upon colonization by pathogenous bacteria. A detailed understanding of the interactions between biological cells and the device surface…

Structure and Recognition in Microbial Membranes Proteins, and DNA

Lead Institution

Oak Ridge National Laboratory

Principal Investigator

T. Straatsma

Project type

Capability Research

Developments in simulation methodologies as well as computer hardware have enabled the molecular simulation of biological systems of increasing size and with increasing accuracy, providing molecular…

Thrust 4: Model Hematite Thin Films and Bacterial Iron Reduction (LDRD: Cytochrome and Whole Cell Interactions with Iron Oxides)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Timothy Droubay

Project type

Grand Challenge

This LDRD project is the fourth one associated with the Biogeochemistry Grand Challenge (BGC). Its overall goal is to develop new capabilities and materials as needed to understand the molecular…

Thrust 4: Whole Cell and Cytochrome Biological Force Microscopy (LDRD: Cytochrome and Whole Cell Interactions wtih Iron Oxides)

Lead Institution

The Ohio State University

Principal Investigator

Brian Lower

Project type

Grand Challenge

This LDRD project is the fourth one associated with the Biogeochemistry Grand Challenge (BGC). Its overall goal is to develop new capabilities and materials as needed to understand the molecular…

Substrate-specific binding of Staphylococcus adhesins to solid surfaces

Lead Institution

The Ohio State University

Principal Investigator

Steven Lower

Project type

Large-Scale EMSL Research

A nearly universal trait of bacteria is their ability to attach to solid surfaces. Attached cells often form biofilms that are implicated in a significant amount of human infections and have been…

Projects