Unraveling the Molecular Biology of Host-Pathogen Interactions
EMSL Project ID
11491
Abstract
Macrophages play an important role in the pathogenesis of Salmonella infection. Critical to the ability of macrophages to kill the Salmonella is the activity of the divalent metal ion transporter natural resistance associated macrophage protein 1 (NRAMP1), a major regulator of host resistance. However, it is unclear how NRAMP1 activity eliminates the Salmonella in macrophages. It is our hypothesis that NRAMP1 activity overcomes the ability of Salmonella to control Salmonella-containing vacuoles (SCV) biogenesis and increases the delivery of antibacterial enzymes to the SCV to kill the Salmonella. To test our hypothesis, we will use different approaches, such as globe proteomic analysis, to examine the effects of NRAMP1 on the SCV biogenesis. Successful completion of this study will to help to achieve our long-term goal of revealing the molecular mechanisms that regulates the host-pathogen interaction.
Project Details
Project type
Exploratory Research
Start Date
2004-10-28
End Date
2006-11-10
Status
Closed
Released Data Link
Team
Principal Investigator
Related Publications
Shi L, SM Chowdhury, HS Smallwood, H Yoon, HM Mottaz-Brewer, AD Norbeck, JE McDermott, TRW Clauss, F Heffron, RD Smith, and JN Adkins. 2009. "Proteomic Investigation of the Time Course Responses of RAW 264.7 Macrophages to Infection with Salmonella enterica." Infection and Immunity 77(8):3227-3233. doi:10.1128/IAI.00063-09