Development of Metabolomics for Biomarker Discovery
EMSL Project ID
12501
Abstract
Metabolomics is a holistic approach to the study of a biological system’s metabolite complement (often generalized to include essentially all small molecules), and its practice is anticipated to greatly impact biomedical research, drug discovery, nutrition science, and clinical practices. The ability to broadly measure metabolites is, in conjunction with gene expression and proteomics data, essential for the delineation of complex cellular networks and pathways. Metabolomics also has great potential utility in clinical settings for rapidly diagnosing disease or characterizing drug toxicity by identifying biomarkers of those cellular pathways altered in the disease state or targeted by therapeutic treatment. This project will focus on developing methods based upon the use of capillary liquid chromatography (LC)-Fourier transform ion cyclotron resonance (FTICR) or high mass accuracy time-of flight (TOF) mass spectrometry (MS) to enable high throughpur, quantitative and broadly comprehensive metabolomics analyses. We will fo this by leveraging the significant separations and mass spectrometry capabilities initially developed at PNNL for proteomics. We will (1) develop the sample processing and separations conditions suitable for metabolomic studies of blood plasma samples, (2) develop initial AMT tag databases of detected plasma metabolites using FTICR- or TOF-MS, and (3) evaluate the quantitative application of the approach using murine blood plasma. This project will complement proteomics biomarker discovery efforts being initiated in conjunction with the Fred Hutchinson Cancer Research Center in Seattle, WA.
Project Details
Project type
Exploratory Research
Start Date
2005-02-25
End Date
2006-01-13
Status
Closed
Released Data Link
Team
Principal Investigator