The Schizophyllum commune proteome
EMSL Project ID
16292
Abstract
BackgroundThe authors, have in parallel with this proposal, submitted a genome sequencing proposal to the DOE Microbial Genome Program (MGP) for Schizophyllum commune (see attached pdf). This mushroom-producing fungus has a rich history, and has been the subject of genetic analysis since the early twentieth century, when Kniep described its’ tetrapolar pattern of sexuality. The seminal work of the Raper laboratory at Harvard in the 1950’s and 60’s laid the genetic groundwork for the molecular characterization of the mating-type genes that continues to this day. S. commune is also used as a model system for studying the genetics of mushroom development, as it belongs to the group of gilled mushrooms that include the commercially valuable species Agaricus bisporus (white button mushroom) and Pleurotus ostreatus (oyster mushroom). S. commune is also emerging as one of the important agents of sinusitis, and is one of the few filamentous basidiomycetes able to colonize and cause severe infections in humans. There are currently laboratories in North America, Europe, and Asia conducting research on both basic and applied aspects of S. commune biology. In addition to its’ utility for examining the molecular genetics of sexual development, S. commune has also been used in studies relating to wood degradation and bioremediation.
Proposed work
We propose collaboration with researchers at EMSL for a comprehensive global proteomic analysis of S. commune. We realize that included in the criteria for acceptance of this application by EMSL would be the acceptance of the sequencing proposal submitted to DOE MGP. Samples from between 3-5 different culture conditions, harvested at 3-5 different time points would be fractionated prior to LC-MS analysis. Thus, we anticipate 200-400 individual samples.The data generated by EMSL would be visualized using the DOE Joint Genome Institute genome browser and displayed on the JGI website. This is similar to a collaboration that is currently underway between the PNNL Fungal Biotechnology group and JGI with the Phanerochaete chrysosporium genome. Finally, we anticipate that the proteomic data generated would be included in the overall S. commune genome annotation publication or as a stand-alone publication.
Project Details
Project type
Exploratory Research
Start Date
2006-02-01
End Date
2007-03-20
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members