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Cerebrospinal Fluid Proteome


EMSL Project ID
18006

Abstract

The exact cause of many neurologic diseases remains unknown. Consequently there are no effective treatments. The cerebrospinal fluid (CSF) is a liquid extension of the brain and is likely to contain biological markers of disease as well as proteomic clues as to the cause of the disease. We propose to do a full proteome characterization of normal CSF, using FTICR and other mass spectrometry facilities at PNNL. This will serve as the roadmap for comparative investigation of disease states. This has never been done before. Once completed we will add CSF samples from patients with firmly diagnosed diseases. One will represent a disease where the initial cause remains unknown- multiple sclerosis (MS) (we will use the earliest identifiable disease known as clinically isolated syndrome or first-attacks). We catalogue both distinct peptides and the quantity of the unique and common peptides many of which may not yet be known to be a CSF component. The second disease will represent a dementia where the initial inciting event is an infection- HIV associated dementia (HAD). As phase 1, the initial AMT databases will be created using pooled samples (200 normals, 5 MS, 5 HAD). As phase 2 we will select 5 samples from each category to assess any relevant variability. Samples will be provided after tryptic digestion of peptides in the sample (this also sterilizes the sample). Samples will be provided in an anonomized fashion.

Project Details

Project type
Exploratory Research
Start Date
2006-04-01
End Date
2007-08-14
Status
Closed

Team

Principal Investigator

Steven Schutzer
Institution
University of Medicine and Dentistry, New Jersey Medical School

Team Members

Joshua Adkins
Institution
Pacific Northwest National Laboratory

Related Publications

Schutzer SE, T Liu, B Natelson, TE Angel, AA Schepmoes, SO Purvine, KK Hixson, MS Lipton, DG Camp, II, PK Coyle, RD Smith, and J Bergquist. 2010. "Establishing the Proteome of Normal Human Cerebrospinal Fluid ." PLoS One 5(6):e10980-. doi:10.1371/journal.pone.0010980