Ultra-High Field NMR Studies of Stable Isotope Applications
EMSL Project ID
2438
Abstract
DNA structure determination by nuclear magnetic resonance (NMR) spectroscopy is an under-restrained problem in general. Isotopic-labeling considerably increases the number and type of NMR experiments that can be collected on DNA samples, and, thus increases the potential number and classes of restraints that can be applied in NMR-based DNA structure calculations. As a result, isotopic labeling allows DNA structures to be better determined by NMR methods and DNA dynamics can be better characterized. Isotopic labeling might be most valuable for the characterization of the structure and dynamics of damaged-DNAs and to our knowledge, no NMR-based damaged DNA structures have been solved with the benefit of isotope labeling. We have synthesized the following 15N,13C-labelled twelve base-pair oligomers (labeled residues are in bold) 5
Project Details
Project type
Capability Research
Start Date
2002-04-01
End Date
2002-11-18
Status
Closed
Released Data Link
Team
Principal Investigator
Related Publications
McAteer K, A Aceves Gaona, R Michalczyk, GW Buchko, NG Isern, LA Silks, JH Miller, and MA Kennedy. 2004. "Compensating Bends in a 16 base-pair DNA Oligomer Containing a T3A3 Segment: A NMR Study of Global DNA Curvature." Biopolymers 75(6):497-511.