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Single Enzyme Nanoparticles


EMSL Project ID
25404

Abstract

Enzymes are useful biocatalysts of nanometer scale that regulate the chemistry of cells and organisms. The specificity of enzymes promises great improvements in various applications such as chemical conversions, biosensing, and bioremediation, trypsin digestion for proteomic analysis, biofuel cells, and drug delivery. However, the short catalytic lifetimes of enzymes presently limit their usefulness. Our goal is to develop new methods for the preparation of nano-bio-composites that provide high yields of enzymes stabilized in the form of single-enzyme nanoparticles (SENs). SENs represent each enzyme particle surrounded and armored by silicate network.1-4 Furthermore, we will immobilize these SENs onto various nanostructured matrices such as nanoporous silica (for bioconversion such as bioremediation).5 These nanostructured matrices will provide a high surface area for the attachment of active and stable SENs. Figure 1 describes these proposed tasks in a schematic way. The combination of stabilized SENs and increased surface area for SENs attachment will result in an ideal enzyme system for applications in biosensors, bioremediation, and other biocatalytic processes.

Project Details

Project type
Large-Scale EMSL Research
Start Date
2007-05-31
End Date
2009-09-30
Status
Closed

Team

Principal Investigator

Jungbae Kim
Institution
Pacific Northwest National Laboratory

Team Members

Ho Nam Chang
Institution
Korea Advanced Institute of Science and Technology (KAIST)

Hyun-gyu Park
Institution
Korea Advanced Institute of Science and Technology (KAIST)

Su Jeong Shin
Institution
Korea Advanced Institute of Science and Technology (KAIST)

Seong Kim
Institution
Pennsylvania State University

Ping Wang
Institution
University of Minnesota

Hyon Bin Na
Institution
Seoul National University

Jay Grate
Institution
Pacific Northwest National Laboratory

Related Publications

J. Kim, J. W. Grate and P. Wang, “Nanobiocatalysis and Its Potential Applications”, Trends Biotechnol. 26(11), 639-646 (2008).
Kim J, BC Kim, D Lopez-Ferrer, K Petritis, and RD Smith. 2010. "Nanobiocatalysis for protein digestion in proteomic analysis ." Proteomics 10(4 SP ISS):687-699.