Soybean Proteomics
EMSL Project ID
30198
Abstract
Soybean is the major source of vegetable oil and, therefore, a major source for biodiesel production. In recognition of this fact, the Department of Energy-Joint Genome Institute recently completed a draft sequence of soybean, with the full genome scheduled for release by the end of 2008. The availability of this genome sequence now enables detailed proteomic analysis in soybean. We propose a collaboration with scientists at EMSL to investigate the proteomics of soybean. Building on current work in our laboratory, the initial focus will be on exploring the proteomic response of soybean root hair cells to infection by the symbiotic, nitrogen-fixing bacterium Bradyrhizobium japonicum. The symbiosis that results is of great agronomic importance due to alleviating the need for expensive nitrogen fertilizer. From the standpoint of basic research, the soybean root hair system also enables the investigation of a single, differentiated plant cell type. We propose to investigate the proteome and phosphoproteome of the soybean root hair cell. Beyond these initial investigations, we would hope to develop collaborations to investigate other aspects of soybean proteomics, including seed development and the response of the plant to stress. Collectively, this work will build a solid platform for soybean proteomics. This will contribute not only to the basic understanding of this plant but its practical uses (e.g., biodiesel).
Project Details
Project type
Large-Scale EMSL Research
Start Date
2008-09-12
End Date
2011-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Brechenmacher L, KK Hixson, M Libault, JT Aldrich, L Pasa-Tolic, and G Stacey. 2012. "Identification of soybean proteins from a single cell type: the root hair." PNNL-SA-86320, Pacific Northwest National Laboratory, Richland, WA. [Unpublished]