Skip to main content

Multidimensional High-Field Magic-Angle Spinning NMR
of Membrane and Nanocrystalline Proteins


EMSL Project ID
30444

Abstract

We aim to extend ongoing studies of membrane and fibril protein structure at the University of Illinois, conducted on 500 and 750 MHz instruments in 3.2 mm magic-angle spinning rotors, to the 900 MHz BioSolids instrumental capabilities at EMSL. Both nanocrystalline model proteins (GB1, DsbA), membrane proteins (DsbB, cytochrome bo3 oxidase) and protein fibrils (alpha-synuclein) are available. High-yield bacterial expression systems for isotopically labeled proteins have been developed at Illinois and initial data acquired. We expect that the resolution and sensitivity at 900 MHz will benefit from the quadratic dependence of sensitivity and resolution of BioSolids experiments demonstrated by preliminary data. This will permit chemical shift assignments to be performed in regions of the spectra that are highly congested at low magnetic field, but well-resolved at 900 MHz 1H frequency. These efforts will thereby provide unique types of data sets not possible at Illinois, which will complement and extend ongoing scientific efforts in the important area of membrane and fibril protein structure determination.

Project Details

Project type
Large-Scale EMSL Research
Start Date
2008-09-01
End Date
2011-09-30
Status
Closed

Team

Principal Investigator

Chad Rienstra
Institution
University of Illinois at Urbana-Champaign

Team Members

Ming Tang
Institution
University of Illinois at Urbana-Champaign

Eric Watt
Institution
University of Illinois at Urbana-Champaign

Kristin Nuzzio
Institution
University of Illinois at Urbana-Champaign

Gemma Comellas Canal
Institution
University of Illinois at Urbana-Champaign

Luciano Mueller
Institution
Bristol-Myers Squibb

Lindsay Sperling
Institution
University of Illinois at Urbana-Champaign

Andrew Nieuwkoop
Institution
University of Illinois at Urbana-Champaign