Multidimensional High-Field Magic-Angle Spinning NMR
of Membrane and Nanocrystalline Proteins
EMSL Project ID
30444
Abstract
We aim to extend ongoing studies of membrane and fibril protein structure at the University of Illinois, conducted on 500 and 750 MHz instruments in 3.2 mm magic-angle spinning rotors, to the 900 MHz BioSolids instrumental capabilities at EMSL. Both nanocrystalline model proteins (GB1, DsbA), membrane proteins (DsbB, cytochrome bo3 oxidase) and protein fibrils (alpha-synuclein) are available. High-yield bacterial expression systems for isotopically labeled proteins have been developed at Illinois and initial data acquired. We expect that the resolution and sensitivity at 900 MHz will benefit from the quadratic dependence of sensitivity and resolution of BioSolids experiments demonstrated by preliminary data. This will permit chemical shift assignments to be performed in regions of the spectra that are highly congested at low magnetic field, but well-resolved at 900 MHz 1H frequency. These efforts will thereby provide unique types of data sets not possible at Illinois, which will complement and extend ongoing scientific efforts in the important area of membrane and fibril protein structure determination.Project Details
Project type
Large-Scale EMSL Research
Start Date
2008-09-01
End Date
2011-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members