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Global measurments of phosphopeptides in human mammary epithelial cells.


EMSL Project ID
3560

Abstract

There is a need to understand fundamental regulatory mechanisms that regulate cell growth and differentiation in breast cells. This information can lead to new routes of treating or preventing breast cancer. The primary goal of this proposal is to identify phosphorylated proteins in human mammary epithelial cells and determine how the profile of these phosphoproteins changes in response to treatment of cells with epidermal growth factor. It is expected that in order to achieve this goal, fundamental methods of detecting phosphopeptides by mass spectrometry will need to be developed and optimized. One procedure to be optimized will be the purification of phosphopeptides using affinity reagents. In addition, the best approach for MS analysis needs also to be determined. It is possible that tandem MS approaches (using an LCQ instrument) may work best for phosphoserine and phosphothreonine while precursor ion scanning techniques (using the QSTARR) may work best for phosphotyrosines. Therefore, these fundamental analytic steps will be developed as part of this work.

Project Details

Project type
Exploratory Research
Start Date
2003-06-27
End Date
2004-07-22
Status
Closed

Team

Principal Investigator

Richard Zangar
Institution
Pacific Northwest National Laboratory

Related Publications

Zaveri R.A., J.E. Shilling, J.D. Fast, and S.R. Springston. 2020. "Efficient Nighttime Biogenic SOA Formation in a Polluted Residual Layer." Journal of Geophysical Research: Atmospheres 125, no. 6:Article No. e2019JD031583. PNNL-SA-146860. doi:10.1029/2019JD031583